Naslov
Lecithin/chitosan nanoparticles for transdermal delivery of melatonin

Autori
Hafner, Anita ; Lovrić, Jasmina ; Pepić, Ivan ; Filipović-Grčić, Jelena

Izvornik
Journal of microencapsulation (0265-2048) 28 (2011), 8; 807-815

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
melatonin; lecithin; chitosan; nanoparticles; transdermal permeability

Sažetak
In this study, the potential of lecithin/chitosan nanoparticles (NPs) as colloidal nanosystem for transdermal melatonin delivery was investigated. Mean diameter and zeta-potential of NPs differing in lecithin type (Lipoid S45 and S100) and chitosan content ranged between 113.7 and 331.5 nm and 4.6 and 31.2 mV, respectively. Melatonin loadings were up to 7.2%. The potential of lecithin/chitosan NPs to enhance transdermal melatonin delivery was investigated by determining the drug flux across dermatomed porcine skin and its skin deposition. Lecithin/chitosan NPs provided 1.3–2.3-fold higher flux compared to melatonin solution. The highest flux, 9.0 ± 0.21 µg/cm2/h, was observed for S45 lecithin/chitosan NPs with lecithin/chitosan weight ratio of 20:1. NP possible cytotoxicity in vitro was evaluated using human skin keratinocytes and fibroblasts. It was demonstrated that lecithin/chitosan NPs can be applied to skin cells at concentrations up to 200 µg/mL without inducing plasma membrane damage or cell viability decrease.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA