Naslov
Evaluation of a molecular fingerprinting method based on the diversity of the ribosomal 16S rRNA gene to describe the dynamic of bacterial communities in Istrian cheese

Autori
Mrkonjić Fuka, Mirna ; Radl, Viviane ; Bogovič Matijašić, Bojana ; Schloter, Michael

Izvornik
African journal of biotechnology (1684-5315) 11 (2012), 101; 16650-16654

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DGGE; 16S rRNA gene; cheese

Sažetak
Denaturing gradient gel electrophoresis (DGGE) is a powerful tool to study bacterial communities’ structure based on the diversity of the 16S rRNA genes. However, the results are strongly dependent on different steps included into the method. Therefore, three primer pairs that amplify different variable regions of the 16S rRNA gene (V1, V3 and V6- V8) were tested in order to investigate the bacterial diversity existent in Istrian cheese. We found that primer set extremely influenced DGGE analysis. V3 primers were most efficient when 15 cheese associated isolates were resolved by DGGE. However, for Istrian cheese analysis the best separation and highest number of bands in DGGE patterns were noticed for V6-V8 primer pairs.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA