Naslov
Effect of spinal and general anesthesia on serum concentration of pro-inflammatory and anti-inflammatory cytokines

Autori
Žura, Marijana ; Kozmar, Ana ; Šakić, Katarina ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, 2011 Annual Meeting of the Croatian Immunological Society / - , 2001

Skup
2011 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rabac, Hrvatska, October 7th-9th 20011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
spinalna anestezija; opća anestezija; citokini
(spinal (regional) anaesthesia; general anaesthesia; cytokines)

Sažetak
The postoperative period is associated with neuroendocrine, metabolic and immune alterations which are the combined results of tissue demage, surgical stress, anesthesia, postoperative pain and psychological stress. Each of these factors affects the immune system, either by enhancing the inflammatory response or suppressing adaptive immunity after surgery. The immunomodulatory effects of surgery, anesthesia and other therapy are difficult to define in surgical patient. In this study, we investigated the effect of spinal and general anesthesia in minor surgery on postoperatively induced changes in immunological parameters. Specifically, we studied the serum concentrations of proinflammatory cytokines (IL-1α, IL-1β, TNF-α, IL-6, IL-8, MCP-1, VEGF, EGF), anti-inflammatory cytokines (IL-4, IL-10) and Th1 helper T lymphocyte cytokines (IL-2, IFN-γ) during perioperative period in 30 patients the American Society of Anesthesiologists (ASA) status I and II who underwent Transurethral Resection of the Prostate TURP) because of benign prostatic hyperplasia. Peripheral venous blood samples were collected 2 hours before surgery, on the first, third and fifth postoperative day. Randox Biochip Cytokine Array was used to perform simultaneous quantitative detection of multiple cytokines from a single patient sample. A sandwich chemiluminiscent immunoassy is employed for the cytokine array. Our results showed that general anesthesia slightly suppressed IL-2 production that peaked postoperatively on day 1 and returned to preoperative values on the fifth postoperative day with significantly higher value in general anesthesia group. In contrast, IL-6 concentrations in both spinal and general anesthesia group increased significantly on the first postoperative day in comparison with preanesthesia levels. IL-6 recovered to preoperative levels on the third postoperative day in both study groups. General and spinal anesthesia also slightly changed the serum concentrations of anti-inflammatory cytokines IL-10 and IL-4. There is no significant difference in serum concentrations of all other cytokines tested between spinal and general anesthesia. In conclusion, the present study demonstrated that spinal and general anaesthesia minimally alter the serum concentration of cytokines during the perioperative period. Altough, these alterations may be of minor importance for subjects with normal immune system changes in cytokine network may be of relevance in patients with preexisting immune dysfunctions.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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