Naslov
Characterization and chromosome location of repetitive DNA in Anemone hortensis L.

Autori
Mlinarec, Jelena ; Papeš, Dražena ; Besendorfer, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XVII International Botanical Congress-Abstracts / - Beč, 2005, 346-346

Skup
XVII International Botanical Congress

Mjesto i datum
Beč, Austrija, 17.07.2005. - 23.07.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Anemone hortensis; satellite DNA; dispersed DNA; fluorescent in situ hybridization (FISH)

Sažetak
Karyotype of the Mediterranean species Anemone hortensis L. consists of three acrocentrics (chromosomes 1, 2 and 3), one meta-submetacentric (chromosome 4) and four metacentrics (chromosomes 5, 6, 7 and 8). In A. hortensis, large AT-rich heterochromatic bands are located intercalary and terminally on long arm of all acrocentrics and terminally on chromosomes 4 and 5. In this study, we report the nucleotide sequences and chromosomal organization of repetitive elements. The tandem repeats isolated after digestion of genomic DNA with EcoRV (JME) and HindIII (JMH2) were 567 bp and 743 bp long, respectively. The JME satellite sequence family was located in all intercalary DAPI bands, while the JMH2 satellite sequence family coincided to terminal DAPI bands. After digestion of genomic DNA with HindIII, 945 bp long JMH1 repeat sequence was isolated. FISH revealed dispersed organization of JMH1 on all chromosomes of A. hortensis. JMH1 exhibited partial homology to the Ty3/gypsy-like retroelement and has to be confirmed as a part of a retrotransposon.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA