Naslov
Illumination of actin cytoskeleton using green fluorescent protein

Autori
Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemica Medica / Suchanek, Ernest - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2000, 120-121

Skup
6th Alps-Adria congress of Clinical Chemistry and Laboratory Medicine

Mjesto i datum
Opatija, Hrvatska, 15.06.2000. - 17.06.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
coronin; actin cytoskeleton; Dictyostelium discoideum; GFP

Sažetak
Coronin is an actin-associated protein originally isolated from Dictyostelium discoideum. It belongs to the WD-repeat family of proteins. Dictyostelium mutants lacking coronin have marked defects in cell locomotion, cytokinesis, phagocytosis and pinocytosis. 5'-end of GFP gene was fused at 3'-end of coronin gene and vice versa. The fused genes were cloned into pDBsrH vector that contains blasticidin S resistance cassette. D. discoideum wild type cells and coronin null cells were transformed with recombinant vector by electroporation. The transformants that expressed GFP-tagged coronin were identified by fluorescent microscopy. Direct localization using GFP-tagged coronin has shown that coronin is found in a variety of dynamic F-actin rich structures derived from the cell cortex. In chemotactic cells coronin is recruited to the leading edge. The defects that characterize coronin null phenotype appear to be partially complemented by GFP-tagged coronin.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA