Naslov
Computational analysis of the relationship between transcription and DNA flexibility in human and fruit fly DNA

Autori
Kokan, Tina ; Vlahoviček, Kristian

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 10th Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation "The secret life of biomolecules", HDBMB2010 / Kovarik, Zrinka ; Varlje, Jadranka - Rijeka : Hrvatsko Društvo za Biotehnologiju, 2010, 154-154

Skup
10th Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation "The secret life of biomolecules", HDBMB2010

Mjesto i datum
Opatija, Hrvatska, 15.09.2010. - 18.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
curvature; bendability; DNA; transcription

Sažetak
The 3D structure of the DNA molecule is not considered as a mere series of regular helical turns creating a helix, but it also possesses many smaller, local polymorphisms, some of which have been found to have a great effect on the behavior of DNA. Multitudinous research efforts have suggested that such polymorphisms play a significant role in important biological processes like DNA packaging, transcription , replication and recombination. Curvature and bendability are two sequence-dependent intrinsic properties of the DNA double helix where curvature is a measure of the torsional flexibility, and bendability is a measure of the anisotropic bending flexibility. The main goal of this research was to observe if there is a specific structural signal in the area of transcription initiation and termination on the genomic level of fruit fly and human DNA sequences. Here we used parts of fruit fly and human known transcript sequences to calculate their respective curvature and bendability profiles and assess the influence that intrinsic flexibility might have on transcription initiation and termination. The overall results show the existence of a distinct signal in the proximity of both transcription initiation and termination sites, as well as signal periodicity, possibly associated with nucleosome positioning.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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