Naslov
Human Xp/Yp telomere analysis by Southern-STELA

Autori
Ivanković, Milena ; Ćukušić Kalajžić, Andrea ; Škrobot Vidaček, Nikolina ; Franić Šimić, Ivana ; Davidović Mrsić, Sanja ; Rubelj, Ivica

Izvornik
Biogerontology (Dordrecht) (1389-5729) 13 (2012), 2; 203-213

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Xp/Yp telomeres ; individual telomere length ; telomere shortening ; cell senescence ; human fibroblasts

Sažetak
Telomeres are specialized structures designed to protect the ends of linear chromosomes. They are dynamic structures such that in normal somatic cells they constantly shorten as cell division progresses. There is compelling evidence that telomere shortening leads to cell senescence, a process perceived as the main cause of aging in higher mammals. Therefore, the features of telomere shortening are of great importance in understanding cell senescence and aging in general. By identifying unique subtelomeric regions, large enough to produce strong chemiluminescent signals, we have provided a new tool for Southern blot analysis of individual human Xp/Yp telomeres. We extend these results with quantitative fluorescence in situ hybridization using peptide nucleic acid probe (PNA Q-FISH) analysis of telomeres on the Y chromosome. Our results demonstrates unequal shortening dynamics between the p and q telomeres.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA