Naslov
Fas deficiency attenuates bone loss during antigen induced arthritis in mice

Autori
Lazić Mosler, Elvira ; Kuzmac, Sania ; Ivčević, Sanja ; Grčević, Danka ; Marušić, Ana ; Kovačić, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arthritis Research & Therapy: 1st Bio-Rheumatology International Congress (BRIC2011) / - London : Delhi : BioMed Central, 2011

Skup
1st Bio-Rheumatology International Congress (BRIC2011)

Mjesto i datum
Tokyo, Japan, 14.11.2011. - 16.11.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
arthritis; osteoblasts; CD95; bone loss

Sažetak
Background Antigen induced arthritis (AIA) is an experimental model of rheumatoid arthritis induced by methylated bovine serum albumin (mBSA) [1]. Hyperplastic synovia in AIA contains fibroblast- like synoviocytes (FLS) with reduced ability to differentiate into osteoblasts, chondroblasts or adipocytes [2]. Since Fas is shown to inhibit osteoblast differentiation [3], we were interested whether such inhibitory effect may contribute to the pathogenesis of AIA. Materials and methods AIA was induced in mice with knocked-out Fas gene (Fas –/–). Three weeks after pre-immunization with mBSA in complete Freund's adjuvant, wild-type (C57BL/6, wt) and Fas –/– mice were injected with mBSA into each knee, whereas controls were injected with equal volume of phosphate buffered saline (PBS). Three weeks after injection we assessed joint diameters, histology, μCT scans, and differentiation of bone marrow- and synovia- derived osteoblasts. Results Knee diameters were increased in mBSA-injected wt mice compared to PBS-injected controls (3.21±0.2 vs. 2.98±0.1, p<0.05, t-test), and this increase was not significant in Fas –/– mice (2.97±0.2 vs. 2.87±0.1). Histology revealed presence of synovial hyperplasia in both mBSA-injected groups, but mBSA-injected wt mice had decreased trabecular bone volume in distal femoral metaphyses (BV/TV) compared to controls (1.08±0.57 vs. 2.55±0.43 ; p<0.05, t-test). There was no significant difference between mBSA-injected and control group in Fas –/– mice (2.34±0.62 vs. 2.61±0.65). μCT analysis showed that mBSA-injected wt mice had decreased BV/TV (2.99±0.19 v. 1.96±0.19 ; p<0.001, t-test) and trabecular number (TbN) (1.03±0.03 vs. 0.64±0.02), as well as increased trabecular separation (TbSep) (256, 89±1395, 12 vs. 312.40±1323.91), compared to controls. mBSA injected Fas –/– mice had deacresed TbN compared to controls (0.815±0.01 vs. 0.64±0.04 ; p<0.05, t- test), with no significant difference in other trabecular parameters. Osteoblast differentiation was increased in both wt and Fas –/– mBSA-injected mice. Conclusions Our study revealed that Fas deficiency attenuated clinical development and bone loss in AIA, and mechanism of this phenomenon needs to be clarified.

Izvorni jezik
Engleski

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