Naslov
Decreased osteoblastogenesis from synovial fluid progenitors as a marker of systemic inflammatory process in juvenile idiopathic arthritis

Autori
Lazić, Elvira ; Jelušić Dražić Marija ; Grčević, Danka ; Marušić, Ana ; Kovačić, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pediatric Rheumatology / Pediatric Rheumatology European Society (PReS) Congress - Briž, 2011, 117-117

Skup
Proceedings of 18th Pediatric Rheumatology European Society (PReS) Congress

Mjesto i datum
Brugge, Belgija, 14.09.2011. - 18.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
juvenile idiopathic arthritis; synovium; osteoblasts

Sažetak
Background: Juvenile idiopathic arthritis (JIA) is characterized by synovial hyperplastic changes, which may contribute to joint destruction by inhibiting osteoblastogenesis. Aims: The aims of this report are: 1) to assess osteoblastogenesis from synovial fluid (SF) progenitors in children with JIA, 2) to assess the effect of SF from patients with JIA on osteoblastogenesis from bone marrow (BM) progenitors, and 3) to assess local and systemic expression of OBL related genes in JIA. Methods: Peripheral blood (PB) samples were obtained from children with oligoarticular JIA (oJIA, n=18), polyarticular JIA (pJIA, n=20), and healthy controls (n=18). SF samples were collected from children with oJIA (n=20) and pJIA (n=7). Osteoblastogenesis was induced with 50 μg/ml ascorbic acid and 5 mmol b-glycerophosphate, in SF cells and BM cells obtained from a healthy donor, and assessed by alkaline phosphatase (AP) histochemistry. Gene expression of Runx-genes, osteoprotegerin (OPG) and receptor activator of nuclear factor-_B ligand (RANKL) was analyzed by qPCR. Results: Osteoblastogenesis from SF cells was higher in children with oJIA, than in pJIA (784.81±216.79 vs. 257.21±68.13 units, p<0.001, t-test), and negatively correlated with erythrocyte sedimentation rate (r=–0.4139, p=0.03). SF from children with oJIA and pJIA inhibited osteoblastogenesis from bone marrow (0.059±0.026 in oJIA ; 0.068±0.019 in pJIA vs. 0, 115 ± 0, 023 in untreated cultures, p<0.05, t-test). Expression of Runx1 and RANKL was higher in SF cells from pJIA than from oJIA patients (p<0.05, Mann-Whitney test). Conclusion: Osteoblast differentiation is locally inhibited in JIA, particularly in children with pJIA, and correlate with systemic inflammatory activity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA