Naslov
Evolutionary optimization of enzymatic reactions by Maximum Entropy Production Principle

Autori
Dobovišek, Andrej ; Županović, Paško ; Kuić, Domagoj ; Bonačić-Lošić, Željana ; Brumen, Milan ; Juretić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts, Regional Biophysics Conference , Primošten , 15-18 September 2010 / - Zagreb, 2010, 31-31

Skup
Regional Biophysics Conference , Primošten , 15-18 September 2010

Mjesto i datum
Primošten, Hrvatska, 15.09.2010. - 18.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
maximum entropy production; enzymatic reactions; evolutionary optimization

Sažetak
A theoretical study of optimization of generalized reversible three state kinetic scheme of enzymatic reaction with respect to the Maximum Entropy Production Principle (MEPP) is presented. We calculated entropy production for each reaction transition of the kinetic scheme as a function either of forward or backward rate constant. We found that for each transition a unique maximum in entropy production exists, and, therefore, the optimal value of the corresponding rate constant could be calculated. Following idea that only second order rate constants are not subjected to evolution1, optimal values of rate constants predicted by MEPP principle are compared to known experimentally determined values for three different types of the enzyme beta-lactamase: beta- lactamase I, PC1 beta-lactamase and RTEM beta-lactamase2. Our results show that MEPP could be an appropriate principle for evolutionary optimization of enzymatic reactions. [1] Heinrich, R., Schuster, S., Holzhutter, H.-G.Mathematical analysis of enzyme reaction systems using optimization principles (1991) European Journal of Biochemistry, 201, 1-21. [2] H. Christensen, M.T. Martin and G. Waley β-lactamases as fully efficient enzymes. Determination of all the rate constants in the acyl-enzyme mechanism, (1990) Biochem. J. 266, 853-861.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

POVEZANOST RADA