Naslov
Early Endosomal Retention of Murine Cytomegalovirus m06 Protein

Autori
Kučić, Natalia ; Ilić Tomaš, Maja ; Mahmutefendić, Hana ; Blagojević, Gordana ; Lučin, Pero

Izvornik
Croatica chemica acta (0011-1643) 85 (2012), 2; 213-221

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
murine cytomegalovirus; m06/gp48; major histocompatibility class I molecules; early endosomal retention

Sažetak
Murine cytomegalovirus (MCMV) encodes several genes which products interact with MHC class I molecules (MHC-I) in the secretory pathway to evade antigen presentation. The product of MCMV-m06 gene associates with nascent MHC-I proteins and redirects them into lysosomes for degradation. This report will demonstrate that MCMV-m06 protein is retained in perinuclear early endosomes together with internalized MHC-I molecules. Since we were not able to capture the MCMV-m06 protein on the cell surface, our data suggests that the MCMV-m06 protein is transported from the Golgi into early endosomal intermediates prior to their maturation into multivesicular endosomes and lysosomes. In NIH 3T3 cells with the transfected m06 gene, the endosomal localization of the m06 protein was not observed, suggesting that another MCMV function remodels the route of m06 trafficking from the Golgi towards late endosomes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA