ࡱ> 5@+bjbj22 42XX <<<<<<<PXXX8,,Pz -//////$dRSQ< S<<  z<< - -<<, hv.|Xmd0|PP<<<<< hc J <  SSPPdMXPPAbstract Submission Form Please forward to abs_hdbmb2010@medri.hr Deadline for abstract submission is July 1th 2010. Name of the presenting author:Natalia Ku iE-mail of the presenting author:natalia@medri.hrType of presentation: place X above preferredxpostershort oral presentationinvited speaker MCMV interference with disposal endocytic pathway of cell-surface MHC-I molecules at early stages of infection Natalia Ku i, Maja Ili Tomaa, Hana Mahmutefendi, Gordana Blagojevi and Pero Lu in Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Croatia Murine cytomegalovirus (MCMV) down-regulates MHC class I molecules (MHC-I) from the cell surface at early stages of infection. Viral interference with both secretory and endocytic endosomal pathways of MHC-I contributes to viral strategy to evade immune recognition. The aim of our study was to investigate the endocytosis of the cell-surface MHC-I in MCMV-infected embryonic fibroblasts and to identify the compartment wherein virus transposes these molecules from the cell surface. Within that the possible role of MCMV m06/gp48 early protein residing in the compartment where internalized MHC-I are accumulated was analyzed. In order to investigate the internalization pathways of down-regulated MHC-I and their accumulation within m06+ retention compartment we used both the internalization assay including cell-surface Abs-labeling after cell-infection with DFcR-MCMV and a palette of endosomal protein markers. Trafficking of Abs-labeled cell surface MHC-I was compared with well known internalization pathways of cell-surface control molecules. The early endosomal compartment was identified by the EEA1 or by the uptake of TfR while Golgi compartment by GM130 and entry of cholera toxin B subunit (CTxB). To determine the MHC-I degradation pathway the EGF and M6PR were used as a control and Lamp-1 for identification of late endosomes/lysosomes. The colocalization experiments were performed by immunofluorescent confocal microscopy. (*02V* D ôzod\d\dQ@ hGhv6B* CJaJphhGh8B* phhJZB* phhGh5LB* phhGhO|B* phhGhvB* H*phhGhvB* ph hGhCB* CJ\aJph hGhO|B* CJ\aJphhGhB* CJaJphhGhB* CJaJphhGh5LB* CJaJphhGh8B* CJaJphhGhMB* CJaJph* F H {Skd$$Ifl0! t644 lad$IfgdCnd$If^ngdv$a$gdv$a$gdM++   $d$Ifa$gd8nd$If^ngdvSkdk$$Ifl0! t644 la     " $ R T r v x  V p ͼͼͼʹ{od\MAMhJZOJQJ^JaJhb7hJZOJQJ^JaJhJZOJQJh\ShJZOJQJh9hJZ5OJQJ$hJZhJZCJOJQJaJnHtH,hJZhJZCJOJQJaJmHnHsHtHhJZhJZCJOJQJaJh>mB* ph hGh85B* CJaJphhGh8B* CJaJphhJZB* phhGh8B* ph hGh86B* CJaJph   $ T t ttttd$IfgdC}kd$$Ifl4\B!` t644 lat v x V  e%*++{{{ttjjb$a$gdJZ $xa$gdJZxgdJZgdJZ}kd$$Ifl4\B!  t644 la J rtz|SuvwRSUW\nux$%%%%%Uh/hJZB*OJQJphhb7hJZOJQJ^JaJhb7hJZB*OJQJphhb7hJZB*OJQJphff%hb7hJZB*OJQJ^JaJphhb7hJZOJQJ^JaJhJZB*OJQJphhb7hJZB*OJQJph4The fate of internalized MHC-I is determined by immunoprecipitation after cell-surface biotinylation following with chase period of 24 hrs. The cell-surface MHC-I are almost completely down-regulated 16 hrs post infection in MCMV-infected cells. Internalized MHC-I are backsorted in endocytic compartments or transported through early endosomes toward Golgi area and accumulated in the compartment where colocalization with viral protein MCMVm06/gp48 was observed. The retention compartment (m06+MHCI+) is accessible and transient for the control molecules (TfR, CTxB, LAMP). In addition, MHC-I and EGF traffic toward late compartments and lysosomes for degradation was maintained but postponed. In the cells infected with MCMV-deletion mutant (Dm06-MCMV) the degradation of cell-surface MHC-I was inhibited, indicating the m06 interference in MHC-I endocytic/degradative pathway. Supported by Ministry of Science and Technology, Republic of Croatia (Grants No.062-0620238-0223 and 062-0000000-3540)      %%d&j&&&&('*'''''''~(((((((\)r)))))))) *(*z*****|++++++ѵфyqjhrdUhGhJZB* phhb7hJZOJQJ^JaJh6JhJZOJQJhJZB*OJQJphh!;hJZOJQJh!;hJZOJQJ hb7hJZB*H*OJQJphhJZOJQJhb7hJZOJQJhb7hJZB*OJQJphhJZB*OJQJph+++++++++++++++$a$gdC dgdC +++++++++++++hGhJZB* phh}hCjlhvUjhrdUhrd 21h:p. A!"#$% i$$If!vh5d5#vd#v:V l t6,5/ i$$If!vh5d5#vd#v:V l t6,5/ $$If!vh5d555#vd#v#v#v:V l4 t6+,5/ / / / / $$If!vh5d555#vd#v#v#v:V l4 t6+,5/  / / / / Dd:\\h  C DA,HDBMB_2010-banner-ENGRrOgsœ%W>FrOgsœ%WJFIFddDuckyPAdobed        !1A"Qaq2BR#br3$ Scs6C4Dt%5uv7W8ᲃEU&VTweF'G !1AQaq"2BRrb#3񒢲S4s5Cc$t6% ?Kz9 hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ'4[[~i^*ORc)c!ԂH!)#,Bo!LeiyJNpj|kg8PI 9֔ЩjE%Y:7o[jq1"M,X /XXЅxcG6LOߕ V2,6R<Gn=V0P{0?'Yt/+G ~!crյuIKu#4M>TQJǎŻ;1DweHʳ5a+ *Tf6_#Cd2bF&q͙JL< g!K-S5'7LEʮ- *ni&tm2w !}b˙VzR GSYr<_?1w?ǣcM=ۧHL[@ [ g卜L;'1x0ݵ+#sdǺsF<ɇ7W:NG[m_Sگq(']K7`J~!CU֯?1o WIC[>C=$ hVۖѥVYf,> uG0VzViH?$A'9yi=̳ro/s|ծњ C#ӕiOMJڕeQiȗ&V;GæV{C$Μ~Q}'ui ~l7h5܁, %zJвp4 JJ@?wHJD ILK(oC+>/nюoU[WvA+ ])*Y.ȩ9UU,@UrIi RB,22J~95xU#!ı:@l[3cTagTVd@BT=|dKWlxJ$W3 hkK$r4&Gb3DP4 6_5}Y<%:\FZSܕpYv&KSOffa ڻ(RU52{]giNq˗~9IM?=STBVvD %K"IL/pX:j>(.%gd@RS2.K+4yj;\uԌl4 Zr9&ø6?k~?-V]c}Mɕdo5cu5JF(ݹ/~[RU %K"ҙ)e;OO:-YMڑR+d,U%e:xnCp<ȹ_ȦV+YU$eM)VdW2ZA_$-lnCH>!ێbz;%~ss;VA*FU# )Ab<4qzI@ıMi ȁ4JYN֏+=~rDFU]$bXZt=<Nv#] `NUy.s~>ݑ?;|U61t})*x] |?K)ᔀ6۾2N)W+Y==dۨI sz`o UbHˮF%,7_)p4'~9G.Tm>1ՔSЭ)n{wiQY;r,Zz2fmJY:u 'JY)o)B#i1er\mIҁ9C`œ;,B24S1FZU1{*Vm1{*]MBTR%ehۺ9Շ~i8T2kq(œԡf^-4fcU$<  D h|jXMhWoe)Fd ZU =wTmiTkHnFiѝ7?ZDVv7:czEAPđSXcԿ{n=- |d&}iY1vilun/ 1>A0 r[-ɸbLnLz1KPT$XtnVE \}GgVMNثt歒1=0n҅Pw鶨.-Td7i^aq[eJϽaR~ν8 `GsXvBu- u*dWY{lZ,vc׽O L'dZ~Y"׫.m*ɆGؠŖyK)̔եc*Ƥy s^U!H_+NY|5 I~zzMͿ%H3SXmA%yWe%$;um\_؞boCb+㽽US丆^\|y 1ĪKީL,n޻Z h-sz^Yr4)Gqe3&J\L~W,@ŽWrXrfij2[+ hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hB8+Wbc]ŻCf4|3Q'?:Vܿ~J{>͇dIVw$!h5l26/(u+UpvEY} 6_+l{cOEPua+RI )[nf8l/Ȳ{Yy+F+!ԍfg*f(2eM TӡѩD9,XɻsZ8mTV ;r>3+trSPpk5޵F<]/] qa䘖Q3f" xMV(%ˇGJ m:yN61e@)toR_6'Jdk>q,R\?*`.?Hǘ`IVctbʡ?*i~{(=p7Ѕk xGHe*A|Nb6`wUC^Fb#p}oBo/T;=2Kf"UK\/%jO]Ȳ#FU?Cc~M)T%i:c0jYf4,'4]ݘYI?Iӻ.i$+RTxNly/.E\9n2>RZr2z* =; \zה)HHlٴ^6I.ָvy[;X/ѰZ߯dmU;ln.c.h/X7*FUL(t[#S r63lm=^ū0C+Gr5I+A$6$TWVX2sbS)ҔkHDɈl{dրĶTܫF|j>=XwOȰSFKl4?aco1Jc Qk*@]A+3"Ғ۪IVl&LOR&'N|:t6-Y86$-;=J4Yk6$i,ݙ3t | %K"Ғ̗e$iɹp?9M-LlzJHJfN49#" RV&Z6&> {qf&s`OױҒ@T. Rȡtd@BTq/px:tG:¥fK;"ҙ)d@BS2 ])*Yl^ˁg};0Fc,Nb\R8NN(>їeĽXwp K ").K)vd:Uu#IaڃڛDfI@S؅Øf>g'?.+¶VJd27''KH ݓd_V?K]t3/e-ygPD(bf24lJؤRS*?$ZCPŒ@9:BFj|" x=h;ҼNa?b24ӽN( VdJD!SGCܣ@p\Z"-v.U;LuV $:P ѐ%cUy_o4bi: $jB@#Ev/PEse#hΔs!YnGi}hSؐ*4Nxv_5 ,G3x$~lz۴ea<،C Q#*䉊I ;A &I1)d洶*Jՙ̟rXث m@&.R1258ҌcҸ*;~7v'Wr&7h5-]}9h%E FáW>e UjYjTsr%OךfմdR:%[D0:S{7Nu1yiZghKEU3|2I;?F&0,m=J`YS{~)jƢ8Gv4A3ViWmwIVҨjFP;yem2f vUyK 1|U oُKmވy=6hy]3ci2L2v $(  SbM*0?p^{Ms %uc  , kߖ>l|+Dd.VfJ|o*&|/koJoՍ$*>RD8DzVđ#+*AVb q*MS0$2،}+6̽p۠o# JP2iZwvq8b/9,!SQ,؅{C+0~ze:Y+Tf 1jkL]ވpubbbz! ~^@iWGoKbtH*ZxMwc iTv{ 6gZ8 THI 7YoƧQ%ˆZ-܏,7jYTVR_%I:" P B>;&g}JMPte]1wZ •Q]ܟv42UЂ?!FpX1. x>| Fs! !wG?zu^0%pǝ2-yy% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% 4\(Xa^vB ٱ_UKV@>WV&Q _~'TS/ 6K-7ؚ \KH*\IfPؚ׀.vHQUeaɈHS/Aʧӽrdۅ0?;~F9{G bcԭ!᜷xXKjVؼwHc)Hbd}g܄o8HAVNe5Ɲ9a1{$H;,hYng YmVB ղXuV:F)Җ"Q8ʙޯT\}rn*[<>*=Ci] !nrmRYH,y$rYܒORIՋ Ғ *\Z#E"I1O JX"C3 d]n̍Y ߽+vYBYG%I'rt:)IrSF[-SVўJǿC$L?!*gIXK:{9Gngp~K5rz^W?ųbKh˜X~I]  pErO9@clWX):;uKy<;ڼOg0]]:d#wW`;eI*:! BS/Rfg tkncx? wTZ~ 8m)*dO t >NbjͻaBU![L[{G{`!ǭ᱉>V{~B}in8cfCubV֜Gb@I`&es9Ir ޾O7nkw.ݠCHJVWGNmP&ZTnP A[ii.%fdPRT ]!)D %J8y$>/rCNFX @6~ .%gd@RTHJfD 2Rr>ƹ%I+N f >W$<`n6-YJixHc_ȫ-?`1-KN)}H\o0H1UIXS4bo+) m/QoX Q҂=wmr̳N]4SPBT2:CǴǶmoQ2[NZA4J|N~%ae&K)j2:G²B u06)귙FŘf;IFIYtJdi 'q)e?Qfi'- 1Fc(h>ziS#.j1)dhk cy&uD[,EԆa|FHċ_XB55&)EY0Rld g!z,4OEj{qܞL,޿؊i)Wsն؍yBT$:jP\)uĭarV_]gȟN3RyPkIu4HvǷrzLKanE_Y<4;STV2IP9QPV{E`&u77cuX.Y('7)?4?U6o k2ٹiGYX=Io=i&袩\V,ʃՊA7YXS&@_NuMV*Ja^J/SQ>c1,R5\H‹Yl xP4fc'R %]>4R[+0zjq?9gzY֚bE<u%2|ӶkC 3 MN% kzZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ$+(_u U βT1ZDŢ@εsVjǫz.Q\ L\XuZf7ǒ.4Yg3na g;^5/ 2*AbHdgaJʤav]˫zz( txO֪-2uF7Jp]NF}Fyv:hCۥgWXIsyU5$Qҩ]%J U+7ҒY8$qIHbrKԹsǸYHt?2 ҭP Ӝhb' 4Ғ^)+ N~"N.C5ߗOJ]u[j#8Gj1)e3L-X#rO}DiWp}9'M8l\sSd*Qۂ#zC Ie1k LdNRR3Gq7Yp,Ia#o5{B5wFS:e!~oD1G}9U9/df3e=86ը‡y3pxDںJSLa6Z.LdH컛49\=L9djE6wz%@$ |dF?Fi Ď\Z-9@Q$oxUs%河!B#:VvTޏABGdn%ʸ GD -6Ԟd2m_#vBר ?':bSƼm GqC.&So<gd(O{}lptޒv)Y# ˺2T3(tZڽL`ޙq*f)O3V;Q e(Ĭj3:\|ɀlN-'E8bh.yP7rb ̱РVXQb1H+<ٙ]jRXژ\*9UVPerB6#k\;woRb:lzG5ZZSJlHulwPv S~AF!s+5 f:./,% *H@baX§mLE)g+#^U3v58F\Ek94+$߯M 1oGC_<7nW Z`1 :mںi.z;TEV.9Tj<֕}`!\NjS4O;v#ԌĞF#!G3c9۾6z)ve>3qeZ.^zBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ+5 J BRTRJJU+4DJvRT%J BT%28k\X*x,߃#jT Ϧ;vF tjf *ăJZ}h@U ',fRl=k՘7 X؂A$VLA,ThR%24q]܅DQ$㾔 -n#r[,)P[-8CPVMRdM2m/GoEVeG$-{uJaJBR"ws*ϻw):gg3AopSy8\RKi^;w9 gU;? GBwIoN3<36nB%T(i L BTS׋T~%xܩ[VUvEmQA ؎m]!+~P `>χl~ +9IM-$ŝݎrI'rN.R ۥ%J(]!*Y.̴pWrty>XTZ1}MS[s]V~U ]))E %J|_qs.=YDt[s)ץ 4"6Z&:3A[!-h؋׷H8 o+ ?h[cǺtQE'?#֊L/ƣ'tl~.XO]_7ۤ50HxDc.zSI}oWsdjm#eFMa5e{5X3*`sL~gZnFon'(:٤6361Pjx+>D'rtzFK}Get[׷ t$ne n6뾐Ҟ?'qȇokO~pȡ 6Қr;TGv8Eh06@CZkܛ_8RzV7Gc/sjy\bE 45)6ْtV=7_:ዃ'q4+F\k = G֠)4Xݾ84zVmW5FسƉ{lM2؞نjB:#cH#I=cݹ%I'UNVnM2` )Xt U(S{!cV\Ӵ~ =:P8CAڬjNNMS.]'{M)_ 4`wmGw&v3n )VZ6oNi&vŬN1XC`mWM6^bZTc- T~HԳ'!-Y)܋bJ:ùX0$ϫS*@ıL}=W+%Z1)eHwGSy5"lZ;_|WQǐs{eU`Lɳ5wl"ٵ5#|A׶CO^am*KB^6,;Y&f)fVC*6vb'/F3K=4,*“=FUL6$'OiSb((l#ԅfYdԷUuP2RОZr :^O,%5=wT2\6&+ Xv}D}q ⾇Ć j2Q^g|7,IdbHĖf'rI=I'A+ 4ZaLZK^efeEoW,@Xov`Mr-j刭"W&J&=Ij +Z#EbRHBUՀ*T>V# !*@DJJe39_6y{1ԣ wI+]QrO@:!*TRB1VwXvqc螖*کQvÐr$epbX!+Njq$B62= 4*(]!+20]!). "dZ|._5v?Ϭ.њjȳP `]~:h[JS;$-?[jV4U]!).(t.ȡtdPBS2(]!*YWHJ` Lȁtd+5rO$w`YwĞ7ڬY[*/"*Նi"׎DVK '2 @Uj⎞d(Jye@#G_6h"VK4BUpZg)c'`Fo5\ :j3e)C߰Jǿ]goޠ'ҀMFۡJÔI⫋b„n+H`,-A^Qجt"Ttƙ-H+zO.ӑ>1)@`lnL0qE{dռ K@!nn!6H|7^_#|UƣXm `ZrW(c)~j;vYz;~[ Iܤ&>Tda4JY&K"&e;Oww/maRdQ8JFX(ܬpB L2 M!]ҙ)eM)V Ud8!dc>1%Z"?vѽX-&ϡXT7f#.zS%,1)e)NCf!sXQJ7:iYGړZñ q{ndH FS;(Y6L4JqT: 9#n䀆70ZKFREZ":>UJ+ܵf܀ -JŎ߻tr>_}?QK+Zq 3Y%I9%K "ѶhCӯF">/ohm#Mpԣ/&LxXvwf|w^kooݻwÎ:{T.Bu8RҀ)@?Y$6$=J %6w XnAL0qoKZ7 ;`ѱWfwpTh:+G˜0Tl|+^;&cacwdn 1:TJ-ǧk.40F/-n lLG綮 'P-Mb,?lݎANXE[@'OS1fd, D"]y-S$t*Y!ְcjzVx1Db2:# Z$2Ա-1ڲziz=?E$mwo1G:Bv2$=]ƺyl^40'e/ |S/NmYThJfl^nkK_s3^uƽu4aYMbX+7xߺ5/,:tbI>\kF S$Gu0ˑ)H؀sYը lo;Kq隒 /nr*YTdw_+2TkҡJYz4E1 R2G z41Z׋*+y6+;sk96̒HѲ ^ֹdkRF$㛁!/DĪZm&Y^Q̧k8:;|BXCh ew5~Sdpg+IF#"Wgns54.b!ktLGL^#c*AɮX>f&9%jm]g\ qԥ9`4in" /.vPtd#2-:ZGeWg#ՏAQcNϕ^POq R<:QqM|rb\wVIBS&%7U+rGg^RŨ-bf)yb݉TPo!8EHiSvIТ$IxސKS<W:8l/3#%2s941-XTm*wP@~"5dR%Sfvg'. ]JC[u)byݾ''&/%&"Omc,<,`rpq0=^lsbEcSD+ed4f}kՈp\V7/8##s' 3FO>w\{7W-ëVdF6xk.>JjWeq<9UrCy^1,kfG:ԡc}k}@P)zoY^k,L Fԓ}O1N;ZfW3RaNxI`h}09˼۪,V^XܕsHQ֎ òԸrp1P\% ,A6.W#_'e!c*%0o)&;6;GRq:FwDT=ӆʞRrn%hWr(_7ȥlc:ZXZKҶ/!^Jo\~?F>X`*cdc(ԌbcdqIفY)'R 1$9+)=kK0Me);C-Ojx^9%MHV%A=,FW#C2T4`dB"&)cudZyzt"c.H\fWV/r.MNsٮ+^+  / Y6G$YP6/ܻT~c5G+9R^#iB4İH!  M-'lS@v&qż*Wrx/E"ӹɒF\7P5$5pLbF0!M%3PJB%f2$q e0JϹG-cx!*Oű=ۑ6Gh{b kU̴I-?&:rBD.᢯|ʴ|dSV(6/۵8Oog9_ay"/;d,قXzJ~)M :$y 5_zyf(e:Zb#1JQ 3Bn9V0BHMpYops9[9 ufim.M\E,q$*="vܖ#괧fElr"%(#3#+##"uS 4iᩀXK4Jw'ywO=~KTzr'R3nXë..+jNyԲy(G13 aP~TeĻɌ)BUiY"ck3{bq {9:|N#ծaJ6q؆R# S=,zRDu}4T✌4p^/T 1ݫ%Ig"4dڱ2l fc'F ZDى8ar'%CZ-xN#c4٤;4Gø@z_5*D .ߴ.y=F !*B iILR p8i_cP̅պ45pO $YF-U 7"5˲74Ϲ1rY*E%uz{5}v멓Ю03b5(7,4SDʇgV%e.,s_4]EejrU2܃_xFiڴAdmP2\2Nf&2Tm'"jhU(N9 jHcI6?Ρ+is(C#E HI#51ĝ+ZqQbB=UFٌ@a66Ww+zsא'mm;xq a(0mɘMTdD 2Rzڕ O4df?jȰN"+f+ S% )߫)kϭb{@ެ dBMf!jchw8;:P 527M~⥌k%%c]Rj@9kRC~߼07oR/4GO)Cfj1$yA+b6'`f;= x=2رG vڨv*J3d#R~\gZӀL6'O929[H,`0QDYB1۶'U4*{3GtT^FŹ'Ѩ5o@B~3j֦oZnw~)Hɜ'{:qw *IJb:xTr(ӂMsC_&/1WЙHb(ջgR(OR8C m/c-=K* P]G"0*ã+t 9Җ(>ca4^%²|XϷH-^YkuSZږgОf_ >٨"ܴq>?rrfjVd1>YXS4{FmLHi7oH7:ф6zĪ‹@7&)4lO]>c3PzeTN8 ~N$aW+dS)!4-RXEȏ|PJZӖ$FδUc`D`GykW3WN$H86V5,G2ya&?f֤ 1I JeԬH@AՐ:I:8icnJ#9}AʜיT6% hBZ1[-Tz7j7}yYmc>c)K0`j!(lp ͆d*Jxgbn=[ôL})N+߀s`bpcYLBbY' 1wLK&&L.8gvCJBu/Odmb)#~wMPLN^B2j61aӄ[xkD+ .ewof/VԵsկ._%J]A^»/_eò ,a.6 poK/yM;(0|͊u0vz}χCܘXc}^{Uߞׇfvgb;$nE$2;:J"@W+rk][c')X.ڍYE|{'JQ HL(@1of\d$9 >zUnV8mJXu1y 6IӥJ4؋p#`R*DI]jrv2G4s1A hDA*L@uR2gx>W9R5ję J2NEpH6QT {NdsC5j oֹVXH3e,{YT:,Nbb)D\` pI3qJDNeג-er Ս$ Q*z~{SIeVIxr[^S4w?i/qXb 4S;C[[ ~ >a;AYpbV@4Z_2{Ko~収\#g2RtG<`luH6M vfT#!go-G<뇾jʸ:ƧZxSR,A[yJt!7'셛hi RBS+y6b*Y{!6Jř5,vHP ,,4@^y,UܭS X~Cӓ`f` &YyWUJ\|kϝDY{X bȈRT)-cgdv_{{w+nn,뾸*r/I@ZB_(LǹK(ږc`0$ԥ-G&|k(&Ǯ:!#}r{C%̼q uؗܫ~"Ჱݥ%l}7gZRBayy9,g\X+u{KUZ6y04f+<4UơT_kt3^\WEJ+J(Xt+VSبd ׍!|Gw7[YWHJ`^X$hdh*pGPA:VKq]ryO0$+Y;+$ͦ2J9i/Q߭gc$YNPTHJde]!*@E N ])*QUt.%0tEUU]!)SPrFb%k!lD>5lNշ^f%K0"+yF(̻7` LB$Ui'IviX%I'$1SDi>Z, _Fwѩ\ z:A(41onĢ: m]{>@{MnO5<AAM]{S]rdmO'ksLr:G34zՔԔ{ڬ-3[6䧆̾,llw٘oԸS,D@ʻx` A,qRGpGURбo~p/ۭŎ5"Rs*}=wد!h"S%bRRԂMnF Ǩel>tX/rKэ ̦}'gqaG}+vkEEX~_: iJ6H[حmew0! ebv>/ ߃+}0!ˢGKr:;jNyjN9GU GC)`F@heEhwd6D{26Y<常"eWl=>u*&ɺGFu֘P-g vw Vlhy{E9.^Cwrvs +ٸ&%`izif0~+1 ShazG$X Vg OihSww^r({NwrD_bkظnUrB׉}`crd, n&-oD@u׽V:FS~Odi< r?[LݽmBY4ѯ݉b4)^IJ\&=@V^iZ% hBe⸾mx\c5S[i/܊U&_Fn:lu~)LFػYOsB*oqD\[I;Z| +3N3پE~3 K"[a?RVD^p7fy427m28ӀyH`Z׹w>`jroqgw zxʷ[,ȒtLtv磜Xs20+ Igxm: VK'xc(E؎8;{噂"1 7b$4 g!y,U-To_=\O_Koڣ_=Ըj̱pSڗ}>5 b1-9$)ZHruf_\ dΕj-KB2?mUO~/zm03nwPFWҢ@g}KټLCd-?oڣeط\O_\ ~F7V5,38WmߢIG<{~ScWCWùWMqpLvF/c}Pw+y0#Fx UXQ zuBZ_k?y}?I.]>v?I,X'7qmTWӠ>Rϙ ņ@y wܿٳ޿h0U/%|_SfDDFZvgebc:mi()V8a';[y̌JY&Bu,7Z%Yx8žgf0bepə@U,K0 Tq2`lLAJyJV?i'){k-I~3үZ6W3vOH,VvjĩTIFn^}-KB^%!~wN=-brb`b$ޜ#zєb(BF2a]ܿ|C1N5)xqDuVO?j.x?'bS2~ߴݚ~<}nBsM+Z@4$XK$Fm2<4XbۂJګ29tt` AZ ıҠ.Ғ *RTҕ(i ȀiT%J8̗qqgpf?zh|Q1Ȫ Me/RP&<ye`jViYU;IzRgX[QZxw ~)0|`ێ[tXkb~hFQ:V-Wɩǜiq⼯9JlU+4fQ5;p:Mi18MՔx:"EC8>jVex,' Ε5Y~ւ!Y]I?zgeN)ى핬E3eϞONzhYZry卛LJv0. 5;ϋkJէ0^`NF%K+66\]mr_8= Qo>ju?x@=[dS[c`1VQlTJ Mp#\CDMltA!Lӥǹ4Zy8]YUfam#$c<*[_l(f l,-0«^{*K궆;\Md'뷞rJ˂a% D!-KBЅ~W]/O%~Rl~K׮_Z߰?}Jϛ't??ԇ_G?yMpx/Ѿzݒ^|-V={ʏUi<>!ֿڣK9eRJh˕rV }>?ɯ%ƽLvm 0q*?*kS/5(IFprU#ZCxHu<KxX|H^@{iYqثdWT+ۉvYr&mجnܜRR@f.S27k̍fݤn{J]Up[d|`{p#mտp|0V1ZdTEzgDZur>S<&az/򐫐5H@"GOBS^|-^E {o\.7/} mC?hosoqUPYFLKcS! 듓Ռz^ύw'Rw?Q_R5}knYE;phr)^9 ql4%f'\|^{,S{I"ʟ< 7ZS ǘ Ŏ־H9JK^|-_@ХF_N|/5R9-bxtkG(&6E 3!}T4U9 u}|aGb?wܧ/_e_kϗ۾wű8܎JXL XTl08vs:4a$AU3H1}$ sq4o֛ՂNxGiPPNZ:1 "P uJ˰4JiJ`J ZBT@4Q(iILH)\>W!csXOG)}иx}JJX^}|_3n( %FG:8htל`;qn, IYU~gl>8n[sc[nT/T5F+g;޻4^[$:AUHQY+P~%2(BS/jr2v6 l#a!7EsڈZ_rH;DEUFFIT\h#S*Ӏ{2@xѾ5 ?m6w-jvv ҩ6lٿjn^{vbI,Y%$%'Y2.W* "%0BS22%0tEUuY)F %2(]!)WUbBePwUVpLmcuiy**2ᩉ`d`J dPBTU䖼؁S@$2/a\D  d-APHHx}+3ڭmqF9*F %0 g$'pnQ]ܬbrOFLc6& .@Bb{@2m 'oW#KYΌ%6 ůb󂔛}?dRC/gYz`Y̐(M!`U4IN_O9z< 8V6=zuZwS#-/T7Xᗧ> ''dۯ5YOS۶Zn#x:0v# SR&;FR3'uQ g} |_/ӗ>ѿfmiٔHMe2C"JYb$iL^a T'l oGmz," |FN˙W,>_%Kdڔ/П_jYu7&_Jb#LJVl+o~HL?&\VF1_=kth=p-1!<(ȁݚGT~u+>OLκ5%8%te>l}lAQđOcR˴eDR*"'haS XvYlT th0->D oD{O@mm*53 .Fv*7DFOVG/Ӹwcx;)m)LyYRGt*ϧ2q!d#*(_,@|&f*evLxZ؝ hVOZRQ2(b55%a ׫f7r^v mz}dk5DL\ wU,9ZB<]a@t sgZS%YLVVl*|f>4xoOJCrT@o$9SD0%CL.-JW'Ԓo,ަ2LEĔIMT$4Jyu hBZ% o_בK/K~l> J_zn~\_ eGW ӈsJfZ~j)jXf;+cčWWp1-sOB"c!&a7G?yM ~zݒ^|-V={ʏUi<>!ֽdWk+:+z< ҺVdYH%Eo, %=+XTN1=`/֟҇9Tߚ+ϋjr ?nuK7zעj3KwЅaا^w}?/#os>_-s)!%lmyҾտ[ 8\ ?c ID̐GOBi%̴Ducb^|-^E {o\.7/} mڞ_=>r|LI=Tl\A-^!,XYԈjmߎ{`޺sXy/ }P4_kkybtyaO=Rq ҷEVHR Ugj)vQ5ȱlk]v?\,9so} pkJ OrsL(r6 ~,ۘxcf>@Refv8QQj\o%X̐<HY8Й *cWCk0VLה-C'te5׆K/HG {?Bq]LO-⭜Y8kɇ{<5R!$,v't٘)R6 7= (f33$+Me;2x5"We5F6죢iB$2Κ'.A+",+dju{L4yS}jD 0ՋpGފ?!Dg=!E)Oj~ ު[ 8o܃$mᨌ r9-LS;8tZn"y>a;qgN"RE!5IS)fUM0O+D26*ۍՀ -[c"ډFv1g%ǩ$n]fJY&3"det%(Aօ 'm)6,j\GV$BW X IZ+*d-IfTm@zG!ąj6J@XuZd) pkU%LW,6Pwo1C̜ ]ub sX7.<ߊR__Sh}aug3T|T= @Fs^X箛%XdEmGN8j~/{r 1)KwH@1=h%~U:vbq7]jl(u1[2u.]C{#7<$ >K'[9Sˤn$q2`Mu "Kf{l;e(dRUN, 42&CncaF,$GoNRj6Iݘj\7yD3S {2@|'60ׇ|;a+bvj;bl<Vs㧠CJkRN IUGRǠ̝r%2i¤HhCoRvgA+UOo ֍ 6闪:Ez_VPY$2MĮᑆfVa0MYZU nɘ/];-G6?P!(LQ?g1LE}ZHjqAzM OC[c6],NՊ+4@&oῨE[5^7옼1{TK[!8q ;+ Ll<؟~OR3bˤj@P9jJtۨ;nwy 1JbF% <'ď%Ir=#OQκ9ytiS^gX?f L&."@s%.Sd=~.>Lbp ٲƷ^fLO2zj1xYT}?JFFu`q_'F9HF;GJ\!y9 ihBZ% o_בK/K~_52e?ߔG/^˵iF[ޏhY_wyO uFh,A6F)byʺ: uNg-HR:q\c*5"2Xm^Iq?C1}a{d9צ_'KB|+u|qZruq3?{x<# Ο3^|}~6EU*7[;~i)B>&n$#yg#@'WvVUYIз@eK"e7hf/',V 3W~_nHY9n peF%#uhBc0Ot?S;qܾURZ{=qt~ BTg/ի 8aQAf$BJ{E˵؏)+ږXHu#ZC`r)$$+r$sA^EOI'1Cos3 [' S k Fz}*5e >nDGM3ߓ7[Blv.D;,}kq]cNl5f(N72|r LK6'{U>p_hp!ӄXJu):8=,SCtC, @F6Јoc <f: .bHb9jWr6tصXu ÂTrk-=<24 <{;ϖ'+1K)`XQft]GYܙLbfFǪ{ṷ̂$-k\Ѽ#^1J:O|Oѥ5I/XFVE 7r[f4aVӚݹn cqr8"'IXU@~ ">j2K,aqȽ^N3:t(┧Un܄E4wْApGz}OH?;{6հBZKzjM>{|n.^ClK1wRz$k9B&g\Pzw!.I#=i4 dldU]XDA4]Z0ƻ"< Q!X\w޵H Q3ORVQz-NWSV oKB;%I#p*DT VJ(26eUj0G18D{vciҨݨ4vWڴ9jߐˌ㬤QHci%{G_r~aR5I4 ,CD{v^їi9D:7u1mj~+pV:f o'OS71 |RxaKuy7tE3?HݑFhj|'TˬvhܱJ]!,(!vqO/JVqH(jp>ɰب-2Tc>'Jse.-Z[YoK@>z/Fb At jLAd⎲45K7" ԑ鲒L6o6RX[M<vB0P~엘|7ḯІ&v륉yԟF1 !GGNT3'c1N21Ry=``b.i|uj(PG {0zꜿo>w1* eU,g{UC%Zw<^\R `:'H7o¬Nu wֵ| #g:\/BqJ7GzGD?}')??A&r/qsK3b,_C|cVc7Y>esy+-P:1cD2 ݡ}*t3R"LpSc1[s=jUNu,QR)":@;ۃ,9yg8uj!S3H Dk؋B9xDV8 bMV=Ȱ_a[mlBC u^~9,nDSG!qMt=4bae/ lz$\ $vp*~g  7ίl1?t.C VICs>Oor^ G,E%RpV8@`WG##Co&_<<Ȍ+IQ*k̤}Fwew _A/?w/D;y#]UWcJTJT(ȃJT((42G[$X }f F 얐l65 P[6Ҥ~ґ(V@JT@40[ sOr>Qms9H?l/S;X.K:R~;e 4 !Rc r |Fg/HFM, 1 I%[NK A'P[$s's]/s() e#MFk^/#R1nգ 10* wh4 q䘨q~bWՕ-\M]eK4RSNxd 21ݘ1{m,,uة8%3Хa&,؝ Ԃ7c= w`7"VF1Cyrq>.{̲E>,k~=8c:e|6Cv(Ѷ]~ˠlU|1ȭ/MH~PDcB!dIrΤ4r(B#]VJeqɞBwS)^3(U/ F?7k3F%0Wg UOH 2uba-YaC­smiR2ټ:Tcp3v?-U4.؆XH+WY;#0vEQYUҭ2EXlRK<_ճ<%RF.uA+;zUh*;\唫R$;,RcbF.w1.n=k^^B@ӕZBݡsz7#ZJy |VRQKtaTX I%45Y)*!)jSUFF%0ʺ#(F%0FUdWHJ`uY)FUdFUdR\Ycq\Vh&rǙA*%CQGP9RU׷9S˃ ҝw:*HRink9[\5L1kqMGCa=I$u=5)ӕI9+ot)`xb~ChG2K ǵU|MջGdժl $kk/^%HnfҫM#+KI#ORu%"KzWU꼳V5x,Wu %]è ҉Ab-^ 9F>$LqUW;-xE1;0Nk^d 5x}YieZF!r2!A~0 %uNOuf'D G.BܱB'W=SINd$LsJ:o q uI}g_g>ߴ[‰oc0G1#}J;ZjY4SGTrudF:ѭK&5H4IE 2LR {oAD*ajWlvv`okvrb֗9W Iv#)dRV;z%Iú6 ?432R5#|H#x.Y"Ì"cZ R_]g=?Aﯲ {T5u;}P #F3/]{za#doDZQH?# |{k7C'D'r7GF ]P=isW+ ;BW +I%rkJ _pmig1(VIRKbYpyG /yJ`1wfwnw''\ T$(ecC/գY0Ezfzze'^ɀ>#]o=7}' $v{6y2qǦP]R.U6N ,BY7'(Ƿē):i-6-^џ2,ҒTH}MN*o)cbCD8vXLh%6@`e~Z-bƣHIbvXlrNrI.VņcEH؝WwׯMv]? ׼\Ṳm2 {\2m#f8Gpq=E;:Vl A }:Rexuʧ^?HRwιk)s+?9_ߗ9BKP^/ٯ(X%Ytai:J@Psu\U:Z?3Mx/cO|^{9i kEbZ@1m+îM:ӧ&忓c#z~^i_Bs,vPj@0K9eI;T3\,W rxHe+sAH@Rcuo D^VST2 l$(Ffvgv,If'rI$J/QȹF^{W^oOLzKuX̹ ӣ^*zq  zN&Xv9;\ҵp9~r.ry/$D%rCrӠ?HY!pK:&R'y%CAUiTҦDRiƔE*Br`MG IOr쉪b&viHyxgpӷ-R#ֳᠬJS"(_nw[ګ33]hxiW+ǽz^ziw]XXC̼R mUrYkg;"@vZNGۨ.c|$7HzzRa9^`I}$ui%U|"'Te1A:;4eyeiga0rԆJJ9$%A2nt|2pfQ ` +w-Ppr68_[=,1E][{]@=ޚXMҐ~#|l+2qYMMAm,m"vkJ~#/ժʾzKʴxNk59>Օ_%-#J~bP[ AU-#as2YՐ;>VNTi deBSUe2e/x.Sboiv&9pـһ+iBQUמ-<|'Bvn>14L~z֜+w17leGE,B,vUI'j?ؚ^W=ʙw(?*Pt„nџ7R0"|Zg jVDu VJdeBSe7ey"||>TKǂT'u,4}"\t?j6/QڳJjS`4FQLjSeBT(d5Y)2VJ`搕(5Y)2VJpi L:%2.Wv֫ wc:,c)F!M &p%|.gɡYc[V=Vyxݲ.(e-*wkۆh Ne;N|s&{Y䒶TR9en6vI ifys.; k+ Zw:Ghبy;{_?M:NnJYSHdBi Hdǔ%0M-b53$3/|6Yw ّ|VSHR,^^ r-PZMʾ?J2'dAY;}==Ṇe3Ðe*ە(JJ$DXje\LHi2Di=>#2 ,^ׯ^ё*V렌4k,39T؝oG9zP j`b01 %;Fk½SPȇk,|oCj.].>#?eP2=XǀeGv+n> P姭JI%X3ԤL u:4c0"D1ዳRIů] Kȑ yY,==rK;E.ǠP7'R %O"`?W^ ?d2&Fh¬Cq̝U331+4٣B7I6r!Q1ٲnVV#B* O@ęeh'5cZQ MVpfAiăZA4?X3PLj^7'eu3'"^zAP4Ǫ2L$ۉV[mJ>T:VXas_!Nex[ *aUhv2G*BwNmtZ9r2o~†Tx4U(WP?H#}sg oޛ E[0*T3 hKMAny2)/_WЄ!-KBЄ!-E&h`VTiQYjm؟%IDb,Vr3i9*p)ȳecFg6]Boqjq?2ىdN2({1DVFB0-dt,[5GSPVI&.PX׽|G9^4cBLʙEi{ ('+7ת\Դ!-KB ^OE*ƹNr+ǯ7nGS/lv:r 8vX St*.*s -˹~5lJ(][ST=P=l|\͹\e+ 1iRC<2)֫  lj&r"xKzО!Dq)(o=zHY YP_/#-ssٓ*EáUuYЄ!-KBYqUyXX cיz858U|GA];87#avۮ:Z% hBؽ`cqȲqa"؏%dz6:W@!YVDP;@7^\k+rCN& _2)N&Y##Y9 opf*לps".er ͎Eg-mepF$ ?B׸d2[#1X֩*]Ekj"-*Ҕ˵(PT "ҕ!i dyMjb"R|I4hK}NX%կ ;~8{Yl:DH;WhD# f~3j/lyמHIQb(%%у RzA{tiYj): ٿC#mnjZnV['UdJSU/&~G;Iu%.ȨȵiKV}jZݐMLr Gk\>ԘrFO'{7frO-jkoÙ$s3u,ӜHV\)ҕHS-7/gnO=zn>3rzAGUni-xU*ve=#J"VTa)Ǽ+fwRi i `EHS`?)y {-#9TQH]\=@/[x _]"pƫd}zaOQzX0BêSM}$ni:|jE󵆕M$M#M4^Y\ff;bzO$j#(dFQ)0 ƳbZQwv-VLv+L#mccQ.PӖ!Fʥv{xds&1V] kR^𲒵q[ZxMXʍg%VTi RjSp5Y)TjTuBSeNpsJΐZ)"*S$F$Qv?@N,# '[NeXJoT_$ZētYr$r;#BI6r#F˵y4YohQN+UL9\${jWJuYД+]n,Rʑ{Ʊ֠k)*2VJ`8,XZDyK,RHw41*2l ɸospY`> "aLyC*\

J/ `5KUw$IqR/[R"C.5}qRXs'uw|@FܪB5)7ۡ~Tˑzpl>Es3p) S; b(s J^lL~ѯx&JdԂlX.7}ppFUN@KhG|+),+rnGR7Tv'jTg>J\?EũU0 t{ q}XH `hcAͼY*V#1Ĭ$;X챓f`t62LLe" 3KE8Sx4a6c+ܠX6w!P%tSkc+R|SV'dK(fu}$?jS\ӝC 0;0Fߩ|jpBK;F+Jvް7>ZBl7v]t# Tgn49| ՇUxy)*N[5S'\LMNaQ3y\*g^)W #róPжrS~$=x> o3LTN 7uǢ'閮Ꟗ2~W>\P1 pN;8YTbh+l*k+5G'i2K:|9R H,֖ӊ-eT'QSH?J4.?uw~ZNJMEx9fuDWzxӑ1%2%俑x%4*PF~TnIt X`---tG2SWdS8*K(gl=Uwgv*:ѧg˽(q8d'24Ջd2H6y2]*ƃs|h&<,ev{vsUiӎ ͼ!Bq$/ 5~}KBЄ!-KBЄ!-KBЄ!-KBЄ!-KBЄ!-KBЄ!-KBЄ!-] ARҕ!t5Jj H" )R4WcJT )RB-c1®{ő`! 8̋Z'1:?lhv`}@9bcNH;Xˑy9+0J%RRA*)TL)$>!cB#ֹ8yTZ# ?*Řf;tW 2Ac\_o7jdyXҜ}:b_ӡvrǗ#LD@y(.3]/ףܷ#'J곘{61Gt8Y#*{XnuQk'7Z+:@qL fq&E?q9 O <"CK^\K՗d{99ol5> jp3 )]#r#Ĥ;$}|.ue@|OMHrlnKJSy/63܎SÎeՀed6'e=}+.ЖZ ҙwRW<"!LF ib Uh,Ve2e`|#q)H.}fyu0aY!QEgH>jgZ>riFGɆh߰h i~y!FPSl[h^9,m6$C 1LKzta3BO9D oݒ62f>j-}Xi̩UwGUwz9rs'Qp'|:75U%_#ʈȑL kFS1T-a-׷VXZ]׻*?1_# 8}Qܽ,BSWQgqH5u0l=J]<ƿ/!gr=)NWkQ;9b;j:Қ:3cb$eNՖ$ |{jդF2.)/1mw l~6O#[f+1,9"CI,05P޳cP7Z3[- f H Y" )B(yCyYoT^R秢\n _2h؂ñس TD4KNZQ_]"lJmf;~XR7ykmc+( D)<ڕ625sz60 ؍=7$ll8`/Ѐlӻ+b֒Oήۢ@nZ 2 v]2$8'x %%}e=HbdH0.#0 pVf`1Hdm7W;22we8#u#5n^xHZcХ4/tb|r8Z0=XW3L?b\V2:f+{l%S#Ѹ>A?TLuYWS 4(9+I R,d*G J1b/2w5 &*`bOY{n 0oFff"|?0Rc MI_~Z8!=N2X,fs#ZݘK1I=~{`'TRW6CIA?ii}CC.r8BT7Qr$ /}vcaM JX)Z) ҒQdc)?O7އO\C+ӅF!_zNlܟi}lxI5V#u?Lw `َWTl|")Lh"6aa:%Vx Q}z4!FGԱs>6?h|H)򅸀rpЈtk!Q5J,q$*U;U$#=7'`7RI>] %Z}GwdQ^qZӊ^ǓZ^1k*% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ% hBZ+*)])R4BJTARBxiJUҔ[呶VK\26?] Q*x}%<$Qs0uHւ(W!ěoqg Y1x>}9Kh|?"[)8/wO}\\ EfZC$aCa~}#jp97㧷?/G9/, cq$8KiM7Fι$ 7ٜ;)Wt|#P1>N8RVD5@U5VrnȿAf)|^4ڣX:х?Xnh#QҺʪZK*{SGMHXԏ}Sy<Bbh)* `~,Xrq6 :b7W]27ub1YNP- 3_-RT"e0GQ`U_8:ʓ21!@KXŪY[XtP ̽Bu5)w_Y9oGz ،wǰq]Jt& XrhĺQD'1FGF (ܒN#X'hT%/xY!qT|O$30*>uNNhTCp}xthupYZuB[RпbJ>$2ːtNڼN'v* 1 ݻ'K8ʔ- e;Ks'J⣮ (%S>"ֿwYӈsR*%E,Ok-gX7%pG붴?`Io2Dot|8ƘYrdyl\KHȤ'&IvN &R2=|RkEkVJqY`< nUK !R˴|2yXE뙐kl4'KΊ>o=f;-VH&u2L2#j$+-,XbWRݶ+okA*7QCo]Pybb>e!u8|>%|v>Y9.<hddV SrP/i.H lpyjǗv&s!̂AKv*2dzw4*?EWTx:?UT5*Ȓz6jdcxձ aާ+ѐ͌fStd ,S,EsSH x].ګ=DԢoy)acL˯KKN4cFcjztbȑ*] .2ʠxz^ *g3t2ِ*s;dv`{"%X*JC@%~cdde.Ne;g UFU@T :kq, ԾgRr#)%2R;E>: )*˂gP=VU=Iܓ'Y tA)HS+nF7qnQ,*J;ۭ(잭)P`~ѱE-YLH^9§1sg*pl]l?jHJuغvI4ҭP"&͡.o~{gL~C<аkW $T`uٻ}gRR:[):P2klZ0|r̝Or/rJEzi]CJYc,G*ϙT}B 7m +20()=yGWHVKzVeuYRF]!RN'ēv?7"_ߌ |-酕~j'`mܧzOxfߴ JU!:4եy^ :WGS؂thZZr?)T ,|?v)<2J| ԙx+`U_{QyR\I5+C1WueY+v?Ȭvw^tx֖yy@bX; *-dsMI/do8XV>$v $҈ï)L=jA=.i $Ѥ3*1<6ur)װlܳBy+U(ÃRqY;RĝSbmbrqլ)9,dx5 m57o͝_FQry} ߲T0;UцᕔV XF& KuARi `W>3g11"Xҳ ߒ7¤~z[J!:(#e8GQ`xYl][ V2v`=:)ӕYFX+#K?n䒶5p.; ONb$31Ejshxb0O/.Ud&˂c]r7Z|? ˬԼOkTnNu!:I!&)c!NH &#Jٍ,Et܁F9(GGwFT ca?Ff*66CGHyח_sUqi7zϨrL, k2(1R=ɥ?d՜>=(d@(0!^I;s]xJ0+׶֔Cf1NzxgEI6v`9$l6:TבpxbJ|gO&RgRoՑt>;k8`>\qxg`FTLe<9[= wc|Y@s?uRףo2e &<[ZXԏNPvoeQ^I:g3'1P̆.Eڮ]B leB}˪ANCQ}I d%J;B+ =U{+ȃ&sIҘ3hHt'pc򸼌V'#Z?XEt/ :wOt&L8[ l"@?G` ؟PĎ µ) ɀ_;K+\~s+zV7N8cuT SѷZ^z%m&<4N]R)4p D15=n ΅~ _!8=X`dZC+6B{]ճYm8 뺨"tQ;x꼷fvI rYMdGECORj'f34S|UЕ`~5R 0҉ DXS58f՗#F %TRO,~GPDBZ'e؏R˯OcC'T"/ 5,F,m"y+6r?T/FÉ|͗%58. P u(ҏԱaPnYUP $ԩʤy:Ȁ/+Rv71*-d;%Cj7뾼TF"w˟oG Wւ`E#Q'pw <*jgm[2LF9[I71nDǓjК1+cX*@{}-<-Fs>mZ:J^Kq G0>>Ѹw-b^P3d&yirwF@FثJ1svW4bZ@gN,Bʻ`~5mG ^,qHoۑcfnu,>۹(dճ")cTpp۳h3_5'ow]>{vv46֭{}=*' H9*`.`rX&ݷug*x**5°9$G^;g zP#*Qx.lR:05}VRN_ݘpr 0&Z %vۮ6ɪ06w^}J/V[urdlzҳ*])j 6w:/i U@/}I-yƖJ:t2(e0FZBX8XI%6l͈AY[UP]_-܁XeR\ǒO9#+ fc,%]e ^߇m-G{U3VFarjUEVdUe0W az o)>{?M7Yo7ߧSm{}f3?fa{ewq~Kyk~ogIu-ӣr^\ٗt``7붵d`%Rd%Bїc+Ɠ%F*+U?X\UU|YN߉yɩc>f>XFd1I:*YP{u)`b8C^˕Р*-B5pQy1 w[k0J"^8GK!%;&I#c"=ڒ"##4(2F `Nޜ+=*גW@,.m׉{!x|K/i^mnߋm[LF${-{/fY=`돵xgDgȥ͏)A=eLxa0tᔕ8䖎fx7I"X>Dv(5gqr+ˢt'`XLc-7*dm=H2JȘ\>i^YmFw<8] ^3ն7aOeNj1˕W|)X[v!e$肶߯]v3ߊu0mCt2suX.L4b`CʡUk9@ޱȪJGr i6 t]FL"Ӊ́;}4~l x.Rt67& t v'i%2+5_!~)L?ꎟxtR )e>ލ }:Msxa!5 pctLY01 [:W~ 3j|Dǿ˾ _:~o=)me2aB['ˈ,sՈTC č*߸2sSK+aq`{P H>!(%1Pۮuj[,02xoPE~Hf2H(  ]Ӈߕ#ݐ؉g҂\w14xڏ2{vzr"8xڏ2vzr/w1O_QSnv0 c/FIT*B*ph 2Buv   *:;<Yeo 0000 0 0$ 0 0 0$ 0 0 0 0 0$ 0 0 0 0 0$ 00@0p@0@0@0@0@0@0 0My00My00My00My00@0@0My000D>o Oy00Oy00 0(  %++   t ++ + 8@0(  B S  ?|k}k#~k#kL#k|"k"k"f;;Q sOXX 8*urn:schemas-microsoft-com:office:smarttagsdateV*urn:schemas-microsoft-com:office:smarttagsplacehttp://www.5iantlavalamp.com/8*urn:schemas-microsoft-com:office:smarttagsCityB*urn:schemas-microsoft-com:office:smarttagscountry-region=*urn:schemas-microsoft-com:office:smarttags PlaceType=*urn:schemas-microsoft-com:office:smarttags PlaceName 120107DayMonthYearknS\eIOY_   -@Ta   " M V ^ c , / 1 5 ~ P e  3;  Natalia-*>mrlGB[>!h.4/E4m?d\G8QJZgacae1~fRhev&Lx}O| F6PM48G [.5LCv8`,s+rd|}&!llO!f{uv   *:; i0@%L PP P@PP$@P$UnknownGz Times New Roman5Symbol3& z Arial7&  VerdanaU.{ @CalibriCentury Gothic5& zaTahoma"1hFTF! ! #4 k@P2qHX $PvZC:\Documents and Settings\zkovarik\Desktop\10th MEETING ON ChE\ABSTRACTS\abstract-form.dotZrinka KovarikNataliaOh+'0x  4 @ LX`hpssZrinka Kovarikrinrinabstract-form.dotdNatalia4taMicrosoft Word 10.0@2~@ @ |! ՜.+,0 hp|  IMI A  Title  !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~Root Entry FpO.|Data 1TableWordDocument42SummaryInformation(DocumentSummaryInformation8CompObjj  FMicrosoft Word Document MSWordDocWord.Document.89q