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Pregled bibliografske jedinice broj: 546112

Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis

Rodrigues, Adriana C.; Garcia, Herakles A.; Ortiz, Paola A.; Cortez, Alane P.; Martinković, Franjo; Paiva, Fernando; Batista, Jael S.; Minervino, Antonio H.; Campaner, Marta; Pral, Elizabeth M. et al.
Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis // Parasitology international, 59 (2010), 3; 318-325 doi:10.1016/j.parint.2010.03.002 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 546112 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis

Autori
Rodrigues, Adriana C. ; Garcia, Herakles A. ; Ortiz, Paola A. ; Cortez, Alane P. ; Martinković, Franjo ; Paiva, Fernando ; Batista, Jael S. ; Minervino, Antonio H. ; Campaner, Marta ; Pral, Elizabeth M. ; Alfieri, Silvia C. ; Teixeira, Marta M.G.

Izvornik
Parasitology international (1383-5769) 59 (2010), 3; 318-325

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Trypanosoma theileri; cistein-proteaze; katepsin-L; stoka; dijagnostika; genetska tipizacija
(Trypanosoma theileri; Cysteine proteases; Cathepsin L; Livestock; Diagnosis; Genotyping)

Sažetak
Although Trypanosoma theileri and allied trypanosomes are the most widespread trypanosomes in bovids little is known about proteolytic enzymes in these species. We have characterized genes encoding for cathepsin L-like (CATL) cysteine proteases from isolates of cattle, water buffalo and deer that largely diverged from homologues of other trypanosome species. Analysis of 78 CATL catalytic domain sequences from 22 T. theileri trypanosomes disclosed 6 genotypes tightly clustered together into the T. theileri clade. The CATL genes in these trypanosomes are organized in tandem arrays of approximately 1.7kb located in 2 chromosomal bands of 600-720kb. A diagnostic PCR assay targeting CATL sequences detected T. theileri of all genotypes from cattle, buffaloes and cervids and also from tabanid vectors. Expression of T. theileri cysteine proteases was demonstrated by proteolytic activity in gelatin gels and hydrolysis of Z-Phe-Arg-AMC substrate. Results from this work agree with previous data using ribosomal and spliced leader genes demonstrating that CATL gene sequences are useful for diagnosis, population genotyping and evolutionary studies of T. theileri trypanosomes.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA

Ustanove:
Veterinarski fakultet, Zagreb

Profili:

Avatar Url Franjo Martinković (autor)

Poveznice na cjeloviti tekst rada:

doi ac.els-cdn.com www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Rodrigues, Adriana C.; Garcia, Herakles A.; Ortiz, Paola A.; Cortez, Alane P.; Martinković, Franjo; Paiva, Fernando; Batista, Jael S.; Minervino, Antonio H.; Campaner, Marta; Pral, Elizabeth M. et al.
Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis // Parasitology international, 59 (2010), 3; 318-325 doi:10.1016/j.parint.2010.03.002 (međunarodna recenzija, članak, znanstveni)
Rodrigues, A., Garcia, H., Ortiz, P., Cortez, A., Martinković, F., Paiva, F., Batista, J., Minervino, A., Campaner, M. & Pral, E. (2010) Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis. Parasitology international, 59 (3), 318-325 doi:10.1016/j.parint.2010.03.002.
@article{article, author = {Rodrigues, Adriana C. and Garcia, Herakles A. and Ortiz, Paola A. and Cortez, Alane P. and Martinkovi\'{c}, Franjo and Paiva, Fernando and Batista, Jael S. and Minervino, Antonio H. and Campaner, Marta and Pral, Elizabeth M. and Alfieri, Silvia C. and Teixeira, Marta M.G.}, year = {2010}, pages = {318-325}, DOI = {10.1016/j.parint.2010.03.002}, keywords = {Trypanosoma theileri, cistein-proteaze, katepsin-L, stoka, dijagnostika, genetska tipizacija}, journal = {Parasitology international}, doi = {10.1016/j.parint.2010.03.002}, volume = {59}, number = {3}, issn = {1383-5769}, title = {Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis}, keyword = {Trypanosoma theileri, cistein-proteaze, katepsin-L, stoka, dijagnostika, genetska tipizacija} }
@article{article, author = {Rodrigues, Adriana C. and Garcia, Herakles A. and Ortiz, Paola A. and Cortez, Alane P. and Martinkovi\'{c}, Franjo and Paiva, Fernando and Batista, Jael S. and Minervino, Antonio H. and Campaner, Marta and Pral, Elizabeth M. and Alfieri, Silvia C. and Teixeira, Marta M.G.}, year = {2010}, pages = {318-325}, DOI = {10.1016/j.parint.2010.03.002}, keywords = {Trypanosoma theileri, Cysteine proteases, Cathepsin L, Livestock, Diagnosis, Genotyping}, journal = {Parasitology international}, doi = {10.1016/j.parint.2010.03.002}, volume = {59}, number = {3}, issn = {1383-5769}, title = {Cysteine proteases of Trypanosoma (Megatrypanum) theileri : cathepsin L-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis}, keyword = {Trypanosoma theileri, Cysteine proteases, Cathepsin L, Livestock, Diagnosis, Genotyping} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Uključenost u ostale bibliografske baze podataka::


  • EMBASE (Excerpta Medica)
  • MEDLINE
  • BIOBASE, Helminthological Abstracts, Protozoological Abstracts, Public Health and Tropical Medicine, Scopus, Tropical Diseases Bulletin

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