Naslov
First identification, isolation in culture and molecular characterization of Trypanosoma (Megatrypanum) theileri in Croatia

Autori
Martinković Franjo, Rodrigues C. Adriana, Ladavac Mirna, Teixeira M. G. Marta, Marinculic Albert

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the XIII International Congress of Protistology / - , 2009, 142-142

Skup
XXV Annual Meeting of the Brazilian Society of Protozoology, XXXVI Annual Meeting on Basic Research in Chagas Disease

Mjesto i datum
Armação de Búzios, Brazil, 23.08.2009. - 28.08.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Trypanosoma theileri; govedo; genetska tipizacija
(Trypanosoma theileri; cattle; molecular characterization)

Sažetak
Typanosoma theileri is described as ubiquitous non-pathogenic trypanosomatid parasite of bovines, despite some reports about its potential pathogenicity. This is the only bovine trypanosome known to exist in Europe. This species is transmitted by tabanid flies and, maybe, also by ticks. Bovine blood samples have been used to supplement culture media employed for routine microbiological diagnoses. Sterility of bovine blood needs to be previously tested and for this purpose in our laboratory we employed blood cultures. This procedure revealed a blood samples that was positive for T. theileri, which has been reported as contaminant in routine bovine blood sterility testing. The culture of trypanosome obtained has been successfully maintained for more than 6 years. Culture smears stained by Giemsa revealed epimastigote forms characteristic of T. theileri. Cultured trypanosomes were used to prepare DNA for molecular diagnosis using a PCR assay based on ITSrDNA, and results confirmed the identification of this trypanosome as T. theileri. In addition, we determined the SSUrDNA sequence of this isolate and aligned its sequence with sequences from T. theileri isolates of bovines from Germany, Brazil, USA and Japan, T. theileri-trypanosomes from water-buffalo, antelopes and cervids, besides a sequence from T. melophagium of sheep keds from Croatia. The isolate of T. theileri from Croatia showed to be highly similar to all bovid isolates, whereas significantly differed from T. melophagium also from Croatia. This result corroborated that although T. melophagium is very closely related to T. theileri, these two trypanosome species circulate in different host species and are transmitted by different vectors, sheep keds and tabanids, respectively. This is the first report of T. theileri in bovine from Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA