Naslov
Occurrence, culture, morphological characterization and phylogeny of Trypanosoma (Megatrypanum) melophagium from sheep keds in Croatia

Autori
Martinković Franjo, Matanović Krešimir, Rodrigues C. Adriana, Teixeira M. G. Marta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Proceedings of the XIII International Congress of Protistology / - , 2009, 301-301

Skup
XXV Annual Meeting of the Brazilian Society of Protozoology, XXXVI Annual Meeting on Basic Research in Chagas Disease

Mjesto i datum
Armação de Búzios, Brazil, 23.08.2009. - 28.08.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
krpuša; Trypanosoma melophagium. morfologija; filogeneza
(sheep ked; Trypanosoma melophagium; morphology; phylogeny)

Sažetak
Trypanosoma (Megatrypanum) melophagium is a nonpathogenic parasite of sheep transmitted by the sheep ked Melophagus ovinus (Hippoboscidae), an obligatory bloodsucking ectoparasite restricted to sheep. T. melophagium is a T. theileri-related trypanosome also restricted to sheep. Sheep keds were detected in fleece of all sheep not treated against ectoparasites from six organic farms in Croatia. A large number of keds were collected, dissected and their guts examined for the presence of trypanosomes by microscopy. Keds were positive for trypanosomes morphologically compatible to T. melophagium, showing epimastigotes in the lumen or attached to the gut epithelium, and rounded metacyclic trypomastigotes. Positive guts generated a culture of trypanosome with large epimastigotes, which was identified by ITS1rDNA-PCR as a T. theileri-trypanosome. Sequence of SSUrDNA was employed to infer the positioning of this new trypanosome in relation to other T. theileri-trypanosome in the phylogenetic tree of Trypanosoma. Although differing from all other T. theileri-trypanosomes, the isolate from sheep keds nested within T. theileri clade, closer to isolates from antelope and cervid than to a cattle isolate of T. theileri from Croatia. Together, host-origin, morphological and molecular data corroborate classification of the trypanosome from sheep keds as T. melophagium. The very low parasitemia in animals infected with T. melophagium prevented the observation of the scanty parasite on smears of blood sheep. We have also performed a survey by IFA for antibodies against T. melophagium in sheep infested with trypanosome-positive keds and in ked-free sheep. Results were all negative, corroborating previous reports about lack of antibodies against T. theileri-trypanosomes in infected bovids. This is the first description of T. melophagium in sheep keds from Croatia, and the first time that an isolate from this species was established in culture and included in phylogenetic studies.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA