Naslov
Cell response to curcumin and cause of curcumin- resistance

Autori
Rak, Sanjica ; Čimbora-Zovko, Tamara ; Gajski, Goran ; Garaj-Vrhovac, Verica ; Dubravčić, Klara ; Batinić, Drago ; Domijan, Ana-Marija ; Sorić, Jasna ; Osmak, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDIR-1 From Bench to Clinic - First meeting with international participation / Sabol, Maja ; Levenat, Sonja - Zagreb : Hrvatsko društvo za istraživanje raka (HDIR), 2010, 74-74

Skup
First meeting of the Croatian Association for Cancer Research with international participation HDIR-1 "From Bench to Clinic"

Mjesto i datum
Zagreb, Hrvatska, 11.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
curcumin ; oxidative stress ; oxidative DNA damage ; malondialdehyde ; glutathione

Sažetak
Curcumin is the major component of the spice turmeric Curcuma longa. It exhibits a diversity of beneficial effects, among them the antitumor activity. However, the complex network of cell response to this natural drug is not yet resolved. Specially the data are missing that could explain the mechanism involved in cell-resistance to curcumin. Using human laryngeal carcinoma HEp2 cells and their curcumin resistant 7T subline, we have investigated the cell response to this drug and molecular mechanisms involved in its resistance. Following the treatment with equimalar doses of curcumin 7T cells (as compared to HEp2 cells), exhibit reduced: accumulation of curcumin, induction of reactive oxidative species (ROS), lipid peroxidation, oxidative DNA damage, the fraction of cells in G2/M phase of the cell cycle, and the induction of apoptosis. The treatment with equitoxic doses of curcumin induced similar effects in both cell lines. However, the induction of ROS, oxidative DNA damage and the fraction of cell in G2/M phase are still lower in 7T cells. These results suggest that the cellular uptake of curcumin and defence of 7T subline against oxidative stress is involved in curcumin– resistance of 7T cells (but this resistance was not due to changes in glutathione or induction of superoxide dismutases (SOD1, SOD2) and catalase expression). These results suggest that curcumin is a molecule that induce a complex network of events involved in cell response, and that resistance to this natural compound is based on multiple factors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA