Naslov
Human decidualized endometrial T lymphocytes do not substantially downregulate CD3 zeta

Autori
Bedenicki, Ivica ; Newton, Darren J. ; Flanagan, Brian F. ; Johnson, Peter M. ; Rukavina, Daniel ; Christmas, Stephen E.

Izvornik
American journal of reproductive immunology (1600-0897) 41 (1999), 4; 245-252

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CD3 zeta ; human ; reproductive immunology ; T-cell receptors ; T lymphocytes

Sažetak
Problem: The T-cell antigen receptor (TCR) has been reported to be down-regulated on T-cells in the decidualized endometrium in early pregnancy. Method of Study: The expression of CD3 zeta a component of the TCR complex, has been investigated in human first-trimester decidual T-cells using flow cytometric analysis of permeabilized cells. Results: Levels of CD3 zeta expression were significantly lower in decidual than in peripheral T-cells from non-pregnant women, as assessed by mean fluorescence intensity (4.2 vs 5.5, logarithmic scale, P<0.05). However, when decidual and peripheral T-cells from the same subjects were analyzed (n=10), mean levels of CD3 zeta were slightly, but not significantly, lower in decidual than in peripheral T-cells (P>0.1). CD3 zeta was not substantially down-regulated systemically as mean cytoplasmic CD3 zeta levels did not differ significantly between peripheral blood T-cells from pregnant women and non-pregnant controls (P>0.2). CD8+ cells outnumber CD4+ cells in decidua, but neither the proportions of these two T-cell subsets positive for cytoplasmic CD3eta nor the mean levels of CD3 zeta were significantly different. Conclusions: These results indicate that human decidual T-cells do not greatly down-regulate CD3 zeta, but it is unclear if a small decrease in mean levels may be sufficient to compromise their capacity for activation.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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