Naslov
International round-robin study on the Ames fluctuation test

Autori
Reifferscheid, Georg ; Maes, Hanna ; Allner, Bernhard ; Badurova, Jana ; Belkin, Shimshon ; Blum, Kerstin ; Brauer, Frank ; Bressling, Jana ; Domeneghetti, Stefania ; Elad, Tal ; Flückiger-Isler, Sini ; Grummt, Hans-Juergen ; Guertler, Rainer ; Hecht, Adiel ; Heringa, Minne ; Hollert, Henner ; Huber, Stefanie ; Kramer, Maike ; Magdeburg, Axel ; Ratte, Toni ; Sauerborn-Klobucar, Roberta ; Sokolowski, Andrea ; Soldan, Premsyl ; Smital, Tvrtko ; Stalter, Daniel ; Venier, Paola ; Ziemann, Christina ; Zipperle, Juergen ; Buchinger, Sebastian

Izvornik
Environmental and molecular mutagenesis (0893-6692) 53 (2012), 3; 185-197

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mutagenicity; genotoxicity; ISO; standardisation; round-robin study; Ames fluctuation test

Sažetak
An international round-robin study on the Ames fluctuation test (ISO 11350:2012), a microplate version of the classic plate-incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by eighteen laboratories from seven countries. Such a round-robin study is a pre-condition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. The laboratories tested four water samples (spiked/non-spiked) and two chemical mixtures with and without supplementation of a S9 mix. Validity criteria (acceptable spontaneous and positive control-induced mutation counts) were fulfilled by 92 to 100 %, depending on the test conditions. A two-step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine-square-root transformation in order to detect significant differences between the test samples and the negative control. A threshold value based on a pooled negative control was then calculated to exclude false positive test results. Statistically positive effects observed by the William’s test were considered negative, if the mean of all replicates of a sample did not exceed the calculated threshold. By making use of this approach the overall test sensitivity was 100 %, and the test specificity ranged from 80 to 100 %.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA