Epigenetic silencing of HNF1A associates with changes in the composition of the human plasma N-glycome

Zoldoš, Vlatka; Horvat, Tomislav; Novokmet, Mislav; Cuenin, Cyrille; Knežević, Ana; Pučić, Maja; Huffman, Jennifer E.; Gornik, Olga; Polašek, Ozren; Campbell, Harry; Hayward, Caroline; Wright, Alan F.; Rudan, Igor; Owen, Katharine; McCarthy, Mark I.; Herceg, Zdenko; Lauc, Gordan

doi:10.4161/epi.7.2.18918

Pregled bibliografske jedinice broj: 544756

Epigenetic silencing of HNF1A associates with changes in the composition of the human plasma N-glycome

Zoldoš, Vlatka; Horvat, Tomislav; Novokmet, Mislav; Cuenin, Cyrille; Knežević, Ana; Pučić, Maja; Huffman, Jennifer E.; Gornik, Olga; Polašek, Ozren; Campbell, Harry et al.

Epigenetic silencing of HNF1A associates with changes in the composition of the human plasma N-glycome // Epigenetics, 7 (2012), 2; 164-172 doi:10.4161/epi.7.2.18918 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 544756 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Epigenetic silencing of HNF1A associates with changes in the composition of the human plasma N-glycome

Autori
Zoldoš, Vlatka ; Horvat, Tomislav ; Novokmet, Mislav ; Cuenin, Cyrille ; Knežević, Ana ; Pučić, Maja ; Huffman, Jennifer E. ; Gornik, Olga ; Polašek, Ozren ; Campbell, Harry ; Hayward, Caroline ; Wright, Alan F. ; Rudan, Igor ; Owen, Katharine ; McCarthy, Mark I. ; Herceg, Zdenko ; Lauc, Gordan

Izvornik
Epigenetics (1559-2294) 7 (2012), 2; 164-172

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
protein glycosylation; plasma glycome; HNF1A; CpG methylation; epigenetics

Sažetak
Protein glycosylation is a ubiquitous modification which affects structure and function of proteins. Our recent genome wide association study identified transcription factor HNF1A as an important regulator of plasma protein glycosylation. To evaluate the potential impact of epigenetic regulation of HNF1A on protein glycosylation we quantified its CpG methylation in 810 individuals. Correlations between methylation of four CpG sites and the composition of plasma and IgG glycomes were analyzed. Several statistically significant associations were observed between HNF1A methylation and plasma glycans, while there were no significant associations with IgG glycans. The most consistent association with HNF1A methylation was observed with the increase in the proportion of highly branched glycans. The hypothesis that inactivation of HNF1A promotes branching of glycans was supported by the analysis of plasma N-glycomes in 61 patients with inactivating mutations in HNF1A, where the increase in plasma glycan branching was also observed. This study represents the first demonstration of epigenetic regulation of plasma glycome composition, suggesting potential mechanism by which epigenetic deregulation of the glycome may contribute to disease development.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
Autori Vlatka Zoldoš i Tomislav Horvat podjednako su doprinjelu radu.

POVEZANOST RADA

Projekti:
119-1191196-1224 - Dinamika kromatina i plastičnost genoma (Zoldoš, Vlatka, MZOS ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Medicinski fakultet, Split

Profili:

Tomislav Horvat (autor)