Naslov
Changes of embryo sensitivity to ABA during seed after-ripening in wheat

Autori
Šarčević, Hrvoje ; Ikić, Ivica ; Maričević, Marko ; Gunjača, Jerko ; Tomasović, Slobodan ; Kereša, Snježana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings / Nyachiro, Joseph - Red Deer, 2011, 54-54

Skup
The 12th International Symposium on Pre-Harvest Sprouting in Cereals

Mjesto i datum
Red Deer, Kanada, 24.07.2011. - 27.07.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
seed dormancy ; embryo sensitivity ; ABA ; winter wheat

Sažetak
The level of seed dormancy at harvest maturity is the main component of resistance to pre-harvest sprouting in wheat. Wheat genotypes with enhanced dormancy show increased embryo sensitivity to exogenously applied abscisic acid (ABA), a phytohormone considered to be involved in acquisition of dormancy. The aim of this study was to determine genotype differences in embryo sensitivity to ABA during the period of seed after-ripening. Twenty four winter wheat genotypes together with a highly dormant check RL4137, were grown in the field at two locations in Croatia during 2008/2009 growing season in a randomized complete block design with two replicates. From each plot 40 spikes were harvested at harvest maturity (approximately 14% moisture content on a wet weight basis). Spikes were hand-threshed and the seeds were stored at 20°C to allow after-ripening. Germination tests were conducted at 20°C in darkness with treatments: whole seeds in water (T1), embryos in water (T2) and embryos in 20 M ABA (T3). Four germination tests were carried out at intervals of 10 days starting from the harvest maturity until 30 days after harvest maturity. Germinated seeds were counted after three and six days and germination rate, expressed as weighted (by day) germination index (WGI), was calculated for each genotype by treatment combination. Combined analysis of variance across locations revealed a significant effect of genotype (G) and location (L) for WGI for all treatments in all germination tests, whereas G x L interaction was significant for WGI for all treatment by germination test combinations except for T2 and T3 at harvest maturity. Generally, at harvest maturity as well as during the course of after-ripening, dormant genotypes showed higher embryo sensitivity to ABA compared to less dormant genotypes. However, large variation in embryo sensitivity to ABA was found within groups of genotypes with high, moderate as well as low level of seed dormancy in all four germination tests at both locations. Furthermore, differences in embryo sensitivity to ABA between dormant and non dormant genotypes disappeared when the two groups were compared to each other at the same level of embryo or seed dormancy attained through genotype dependent time of after-ripening. In summary, results of the present study indicate that at least part of the differences in wheat embryo sensitivity to ABA during the period of seed after-ripening is the consequence of dormancy status of a seed sample regardless of its genotype (dormancy per se).

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA