Naslov
Serotonin and immune response : effect of the amine on in vitro proliferation of rat lymphocytes

Autori
Štefulj, Jasminka ; Čičin-Šain, Lipa ; Schauenstein, Konrad ; Jernej, Branimir

Izvornik
Neuroimmunomodulation (1021-7401) 9 (2001); 103-108

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
serotonin; 5HT; lymphocyte; proliferation; mitogen

Sažetak
Objective: The effect of serotonin (5-hydroxytryptamine, 5HT) on in vitro proliferation of mitogen-stimulated lymphocytes was studied in primary cultures of rat spleen cells. Methods: 5HT was added to the cultures 1 hour prior to the mitogen, in final concentrations from 10-13 up to 10-2 M. T and B cell mitogens (ConA, PWM, LPS) were used in suboptimal and optimal concentrations. The cell proliferation was measured 24-72 hours after the addition of mitogen. The effect of each 5HT concentration was studied on a group of 6-12 animals and was expressed as percentage of control values obtained with mitogen alone. Results: No significant effect of 5HT in concentrations from 10-13 to 10-5 M was found. At concentrations of ł10-4M a regular dose-dependent inhibition of the lymphocyte proliferation appeared, the concentration producing the half-maximal effect (IC50) being 6x10-4 M. The observed suppression was not due to 5HT cytotoxicy toward spleen cells. Conclusion: Thus, with the experimental system used, we failed to confirm an immunostimulatory effect of 5HT in the range of concentrations of its receptor sensitivities or lower, but found a clearcut immunoinhibitory effect at higher 5HT concentrations.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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