Naslov
Renal type II Na/Pi-cotransporter is strongly impaired whereas the Na/sulphate-cotransporter and aquaporin 1 are unchanged in cadmium-treated rats

Autori
Herak-Kramberger, Carol Mirna ; Spindler, Benjamin ; Biber, Juerg ; Murer, Heini ; Sabolić, Ivan

Izvornik
Pflügers Archiv (0031-6768) 432 (1996), 2; 336-344

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
proximal tubule ; brush-border ; inorganic phosphate ; sodium-phosphate cotransport ; sodium-sulphate cotransport ; water channels ; cadmium nephropathy ; nephrotoxicity

Sažetak
The cellular mechanisms of cadmium (Cd) nephrotoxicity are poorly understood. In this study we investigated cellular causes of the Cd- induced phosphaturia in rat. Compared to controls, Cd-treated rats (2 mg Cd/kg B.W., s.c. for 14 days) showed a marked polyuria, proteinuria and phosphaturia. As studied by the rapid filtration technique in isolated cortical brush-border membrane vesicles (BBMV), Na+ gradient-driven uptake of phosphate (32Pi) and of 3H-glucose were markedly decreased in Cd-treated rats, whereas uptake of sulphate (35S) remained unchanged. By Western blotting of BBMV proteins and by indirect immunocytochemistry in 4 um frozen fixed kidney sections, using an antibody against the type II Na/Pi-cotransporter (NaPi-2), we found a diminished expression of this protein in brush- border membrane from Cd-treated rats. However, the expression of water channel aquaporin 1, estimated from the specific antibody staining in brush- border membranes, remained unchanged by Cd. Northern blot analysis showed a strong reduction of 2.7 kb NaPi-2-related mRNA in Cd-affected kidneys. Our data indicate that (a) Cd may reduce reabsorption of Pi in proximal tubules by affecting the expression of the functional Na/Pi- cotransporters in the luminal membrane, and (b) Cd effects on brush-border transporters are selective.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA