Naslov
Ultrasensitive determination of beta-carotene in fish oil-based supplementary drugs by HPLC-TLS

Autori
Luterotti, Svjetlana ; Franko, Mladen ; Bićanić, Dane

Izvornik
Journal of pharmaceutical and biomedical analysis (0731-7085) 21 (1999), 5; 901-909

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
beta-carotene determination; fish body oil preparations; HPLC-TLS

Sažetak
This research study demonstrates that thermal lens spectrometry (TLS) combined with efficient isocratic HPLC separation provides an excellent analytical means to profile and quantify carotenes in fish body oils and possibly also in vegetable oils. In particular, a highly sensitive, selective, simple and rapid method for the determination of ultratraces of beta-carotene in fish oil-based supplementary drugs has been proposed. The analyte could be determined reliably and precisely in the presence of a complex matrix including other fat-soluble vitamins (A, D and E), polyunsaturated fatty acids, sterols and other pigments by matrix-matched calibration in cod liver oil. The suitability of the method is also evidenced by favourable analytical figures of merit for beta-carotene such as a linearity range of 1-120 ng/ml, LOD of 0.58 ng/ml, recovery of 101.4 ± 3.3% and measurement repeatability of 4.1%.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Farmacija

POVEZANOST RADA