Naslov
Experimental ischemic stroke: changes in lipid peroxidation and antioxidant enzyme activities in rat cortex

Autori
Mršić-Pelčić, Jasenka ; Pilipović, Kristina ; Vitezić, Dinko ; Župan, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Osredkar, D. ; Koritnik, B. ; Pelko, M. - Ljubljana : Neuroscience Association, 2011, 147-147

ISBN
978-961-91704-4-1

Skup
SiNAPSA Neuroscience Conference ´11

Mjesto i datum
Ljubljana, Slovenija, 22.09.2011. - 25.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
focal cerebral ischemia; lipid peroxidation; SOD; GSH-Px; cortex; rat

Sažetak
In the present study, we examined the effect of middle cerebral artery occlusion (MCAO) on lipid peroxidation and antioxidant enzymes (superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in rat cortex. Right MCAO was induced in male Hannover Wistar rats (250-350 g) for 1 hr. After 23 hrs of reperfusion, ischemic animals were sacrificed and the levels of lipid peroxidation, SOD and GSH-Px activities were determined spectrophotometrically in the right and left cortex. Sham operated animals served as the control group. Focal cerebral ischemia significantly increased the level of thiobarbituric acid-reactive substances (TBARS) in the right cortex in comparison to the control group while SOD and GSH-Px activities were not affected. MCAO did not cause contralateral cortical damage since TBARS levels as well as antioxidant enzymes activities were not statistically different in the left cortex. Our results showed that transient focal cerebral ischemia caused oxidative lipid damage in the right cortex of rats after 23 hrs of reperfusion without affecting antioxidant enzyme activities. Changes in activities of above mentioned parameters in left cortex did not reach the level of significance.

Izvorni jezik
Engleski

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