Naslov
Apyap1 affects aflatoxin biosynthesis during Aspergillus parasiticus growth in maize seeds

Autori
Reverberi, Massimo ; Zjalić, Slaven ; Punelli, Federico ; Ricelli, Alessandra ; Fabbri, Anna Adele ; Fanelli, Corrado

Izvornik
Food additives and contaminants (0265-203X) 24 (2007), 10; 1070-1075

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aspergillus parasiticus ; aflatoxins ; lipoperoxides ; maize

Sažetak
It is demonstrated that, in fungal cells grown in synthetic media, the Apyap1 gene is implicated in the modulation of aflatoxin biosynthesis following the perturbation of redox balance. This study suggests that an association between oxidative stress and aflatoxin biosynthesis also occurs in maize seeds. We used DeltaApyap1, a strain in which the gene Apyap1 was disrupted, to verify whether this oxidative stress-related transcription factor, by affecting cell redox balance, can have a role in the modulation of aflatoxin synthesis. The amount of hydroperoxides (ROOH) produced by wild type (WT) and DeltaApyap1, both grown in potato dextrose broth, was assayed in the filtrate. In maize seeds (30 g), inoculated with WT and DeltaApyap1conidia and incubated at 30 degrees C for 15 days, lipoxygenase activity (LOX), lipoperoxides (LOOH) production, fungal growth and aflatoxin biosynthesis was analysed. It was observed that DeltaApyap1 released more hydroperoxides in the culture media and more aflatoxins in seeds, possibly through stronger stimulation of LOX, which, in turn led to greater LOOH production in the seeds. On the basis of the results, a hypothesis regarding strategies to control aflatoxin synthesis is formulated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

Napomena
Rad je prezentiran na skupu MYCOGLOBE Conference
"Advances in Genomics, Biodiversity and Rapid
Systems for Detection of Toxigenic Fungi and
Mycotoxins", održanom u Bari, Italija

POVEZANOST RADA