Naslov
Sgs1 controls the fidelity of gene targeting

Autori
Štafa, Anamarija ; Miklenić, Marina ; Lisnić, Berislav ; Svetec, Ivan-Krešimir ; Zgaga, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, ostalo

Skup
Genome Integrity Discussion Group, The New York Academy of Sciences, Petrini J., Rothstein R., Symington L.S. ur, New York, SAD

Mjesto i datum
Sjedinjene Američke Države, 06.06.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
ends-out recombination; chromosome duplication; Saccharomyces cerevisiae

Sažetak
Introduction of linear, non replicative DNA fragments is widely used for the replacement of homologous sequences in the yeast genome. Moreover, it is studied as a general model for genetic manipulations by ends-out recombination. It is generally assumed that, in contrast to higher eukaryotes, transformation of the yeast Saccharomyces cerevisiae with non replicative DNA occurs almost exclusively by homologous recombination. We found out that the ends-out recombination may result in different genetic alterations in the yeast genome (1). Three clases of events were identified: illegitimate integration, integration of the transforming DNA next to the targeted region and disomic, heteroallelic transformants containing starting allele and allele expected after succesful gene targeting. We performed a systematic study of the influence of type of gene modification (insertion, replacement and deletion) on the accuracy of ends-out recombination.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA