Naslov
Chemokines and metalloproteinases gene expression in early infection of MRC-5 cells with Mycoplasma pneumoniae

Autori
Tadin, Ante ; Matijević, Ivana ; Antonović, Ivan ; Pintera, Alojzija ; Jug, Renata ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - 2009 Annual Meeting of the Croatian Immunological Society / - , 2009

Skup
2009 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Starigrad, Hrvatska, 01.10.2009. - 04.10.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Chemokines; metalloproteinases; MRC-5 cells; Mycoplasma pneumoniae

Sažetak
Bacteria Mycoplasma pneumoniae causes tracheobronchitis and atypical pneumonia in humans. Important component of M. pneumoniae immunopatogenesis is induction of proinflammatory chemokines secreted particularly by lung macrophages and endothelial cells. However, no information is available about the role of lung fibroblasts in infection with M. pneumoniae. In our study we investigated the ability of M. pneumoniae to induce gene expression of chemokines IL-8, MCP-1, MIP-1β and metalloproteinases 2 and 9 (MMP-2 and MMP-9) in human lung fibroblast like MRC-5 cells in early phase of infection. Additionally, we looked for immunomodulatory effects of antibiotics which are commonly used in the treatment of atypical pneumonia caused by M. pneumoniae. For that purpose, MRC-5 cells were infected with M. pneumoniae and also treated with antibiotics azithromycin, ciprofloxacin and doxycycline. Two time points, two and six hours after infection were checked. After isolation of mRNA and synthesis of cDNA, detection of sequences specific for chemokines and MMPs was performed by real time PCR (Roche Applied Science LightCycler System). The most striking effect of M. pneumoniae on MRC-5 cells was induction of IL-8 expression. MCP-1 and MMP-2 genes did not show significantly higher expression after infection with M. pneumoniae and MRC-5 cells showed not to express MIP-1β and MMP-9 genes. Azithromycin, ciprofloxacin and doxycycline showed to have immunomodulatory activity. Azythromycin enhanced expression of MCP-1 and all three antibiotics enhanced expression of IL-8 in the initial phase of infection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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