Naslov
Development of multi-residue analytical method using liquid chromatography tandem mass spectrometry for pharmaceuticals determination in water samples

Autori
Zrnčić, Mirta ; Petrikovski, Aleksandra ; Babić, Sandra ; Kaštelan-Macan, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th International School of Ion Chromatography / Ukić, Šime ; Bolanča, Tomislav - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2011

ISBN
978-953-6470-54-9

Skup
12th International School of Ion Chromatography

Mjesto i datum
Zagreb, Hrvatska, 30.06.2011. - 01.07.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
liquid chromatography; mass spectrometry; pharmaceuticals; multi-residue method

Sažetak
Pharmaceuticals cover a wide range of different compounds and are used to cure disease and fight infection both in humans and in animals. Their occurrence in the environment has become an issue of great importance in the last decade. Because of their wide consumption they have been used in large quantities for years and for the last few decades they have been investigated as a new type of so called emerging contaminants. In the environment they can be found in low concentrations of μg/L or ng/L but their continuous input can pose a potential long term risk for aquatic and terrestrial organisms as well as for human health. [1] To detect and quantify pharmaceuticals in the environment is very complicated because the matrices in which they occur are complex, they have very diverse physicochemical properties and they are found in very low concentrations. Therefore it is of great importance to develop analytical methods that will be highly sensitive and selective in order to monitor pharmaceuticals in the environment. [2] Liquid chromatography coupled to mass spectrometry especially multidimensional mass spectrometry (LC-MSn) is a powerful technique which can provide adequate specificity and sensitivity. [3] The aim of this work was to develop a SPE-HPLC-ESI(+)-MS/MS analytical method for determination pharmaceuticals in wastewater. Investigated pharmaceuticals belong to different classes of antimicrobials: sulfonamides (sulfaguanidine, sulfadiazine, sulfamethazine and sulfametoxazole), a sulfonamide synergist from diaminopyrimidine class (trimethoprim), fluoroquinolones (ciprofloxacin, enrofloxacin and norfloxacin), macrolides (azithromycin, erythromycin, clarithromycin, roxythromycin, tylosin, azahomoerithromycin), tetracyclines (oxytetracycline, tetracycline), antihelmintics (febantel, levamisol, praziquantel), anestetics (lidocain, procain), a steroid hormone (dexametazone) and antibiotic (tiamulin). Sample preparation was done by solid phase extraction (SPE) and the pharmaceuticals were extracted from water using optimized SPE system. Chromatographic separation was done on HPLC – MS/MS Agilent 1200 and the detection on Agilent 6410 triple quadrupole mass spectrometer equipped with electrospray ionization (ESI). Mobile phase was 0.1% formic acid in water and 0.1% formic acid in acetonitrile in gradient mode and the chromatographic column was phenyl Synergi Polar 2, 5μm, 100 x 2.0 mm (Phenomenex)

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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