Naslov
Fusarium graminearum from non-agricultural hosts in Croatia

Autori
Poštić, Jelena ; Leslie, John F. ; Ćosić, Jasenka ; Jurković, Draženka ; Vrandečić, K. ; Saleh, A.A.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Edward Arseniuk, Elzbieta Czembor, Tomasz Goral - Radzików : Plant Breeding and Acclimatization Institute, 2010, 197-197

ISBN
83-891172-44-5

Skup
11th European Fusarium Seminar

Mjesto i datum
Radzików, Poljska, 20.09.2010. - 23.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Fusarium graminearum ; native plants ; weeds ; plant debris

Sažetak
Fusarium graminearum is the most common causal agent of Fusarium head blight of small grains worldwide and is one of the most intensively studied fungal plant pathogens. Fusarium graminearum can overseason in the form of perithecia, mycelia or macroconidia. We isolated Fusarium samples from native plants, weeds and plant debris at 12 locations in Croatia. Of 370 isolated Fusarium strains 65 were identified as F. graminearum. These strains were recovered from maize, wheat and oat field debris, and on living plants of Abutilon theoprasti, Sorghum halepense, Amaranthus retroflexus, Chenopodium album, Arctium lappa, Agrostema githago, Medicago falcata, Xantium strumarium and Capsella bursa- pastoris. Morphological identification was based on mycelial characters of the mycelia from the PDA culture, and the presence/absence and morphology of micro- and macroconidia and chlamydospores from the CLA culture. Mycelia growth rate, color and density of mycelia growing on PDA also were recorded. For molecular identification DNA was extracted from cultures grown in shake flasks with a CTAB protocol. Initial molecular identifications were done with Amplified Fragment Length Polymorphism protocol. The AFLP fingerprints were based on restriction digests with MseI and EcorI and scored manually. Out of 65 isolated samples of F. graminearum, 26 were from maize debris, 18 from wheat debris, 5 from oat debris, 4 from A. retroflexus, 3 each from S. halepense and C. album, 2 each from A. githago and M. falcata, 1 each from X. strumarium, A. theophrasti, C. bursa-pastoris and A. lappa. Native plants and weeds had no obvious disease symptoms. Thus, it is possible to recover F. graminearum not only from the debris of cultivated plants, but also from native plants and weed species in or associated with important agroecosystems in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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