Naslov
DNA polymorphisms in austrian and dalmatian black pine

Autori
Liber, Zlatko ; Fulgosi, Hrvoje ; Pavletić, Zinka ; Nikolić, Toni

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
25. Tagung der Ostalpin-dinarische Geselschaft fuer Vegetationskunde - Abstracts / Huebel, Erich - Beč : Institut fuer Botanik der Universitaet fuer Bodenkultur, 1997, 1-1

Skup
25. Tagung der Ostalpin-dinarische Geselschaft fuer Vegetationskunde - Wien

Mjesto i datum
Beč, Austrija, 04.07.1997. - 08.07.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
black pine; cpDNA; DNA polymorphisms; Pinus nigra; RAPD; RFLP; molecular systematics

Sažetak
Dalmatian black pine with its morphological characteristics essentially differs from other species of black pine complex. The systematics status of Dalmatian black pine is still unexplained and is even considered as a special species: Pinus dalmatica Vis.. The plants from heterogenous habitats were examined: Austrian black pine from Botanical garden Zagreb, Dalmatian black pine from mountain of Biokovo and Dalmatian black pine from the island of Hvar. The methods of chloroplast and total cell DNA isolation were used in this work. The results of RFLP analysis of chloroplast DNA showed that there is no difference in restriction fragments among these investigated plants. Since chloroplast DNA is evolutionary conservative DNA, we can draw a conclusion that all examined plants probably belong to the same species. The RAPD method which is the most appropriate for plant systematic at the species level and below, has shown DNA polymorphisms between and within investigated populations and also distinctly separated Austrian and Dalmatian black pine. The results support the subspecies level for Dalmatian black pine within Pinus nigra complex as the most appropriate. RAPD method demonstrate higher similarity between Austrian black pine with Dalmatian black pine from Biokovo than Austrian black pine with Dalmatian black pine from the island of Hvar and remind us to an earlier hypothesis that the black pine from Biokovo represent an intermediate population.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA