Naslov
Expression of placental antioxidant enzymes is reduced in type 1 diabetes during early pregnancy

Autori
Hiden, Ursula ; Gutschi, E ; Ivanišević, Marina ; Đelmiš, Josip ; Desoye, Gernot

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 43rd annual meeting of DPSG / Simmons, David ; Dornhorst, Anne ; Murphy, helen ; Temple, Rosemary - Cambridge : DPSG, 2011, 14-15

Skup
The 43rd annual meeting of DPSG

Mjesto i datum
Cambridge, Ujedinjeno Kraljevstvo, 22.09.2011. - 24.09.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
placental antioxidant enzymes; tye 1 diabetes; pregnancy

Sažetak
Objectives: Increased reactive oxygen species (ROS) production in diabetes causes increased oxidative stress. Consequently, gestational diabetes (GDM) increases expression of antioxidant enzymes in the placenta in the 3rd trimester. Whether the placenta compensates diabetes-induced oxidative stress also in the 1st trimester is unknown. Therefore, we determined expression of antioxidant enzymes as well as of ROS producing enzymes xanthine oxidase (XO) and NADPH oxidases 4 and 5 (NOX4, 5) in 1st trimester placentas from Type 1 diabetic (T1D) pregnancies compared to healthy pregnancies. Methods: 1st trimester placentas from normal (n=14) vs. T1D (n=16) pregnancies between gesta-tional week 6-12 were obtained after pregnancy interruption for psychosocial reasons and immediately frozen in liquid nitrogen. Total RNA was isolated, and mRNA expression of superoxide dismutases 1-3 (SOD1-3), glutathione peroxidases 1, 3, and 4 (GPX1, 3, 4), catalase (CAT) and glutathione reductase (GSR) measured by real time PCR. Also, expression of enzymes producing ROS ie, NOX4, 5 and XO was determined. Data were normalized to expression of the housekeeping gene HPRT1. Results: Expression of SOD2 was decreased by 38% (p=0.036) in T1D placentas from week 8-12, but not from week 6-7. GPX3 was reduced by 63% (p=0.006) in ali investigated gestational weeks. GPX1, GPX4 and CAT showed also lower expression in T1D placentas between weeks 8-12 but did not reach significance. Expression of the ROS producing enzymes NOX4, 5 and XO was unchanged. Conclusion: This is the first study measuring diabetes associated changes in placental antioxidant enzyme expression in early pregnancy. In contrast to GDM in the 3rd trimester, we found a down-regulation of placental antioxidant enzymes in the 1st trimester. This suggests that either 1) oxidative stress is reduced in T1D 1st trimester placentas causing reduced antioxidant enzyme expression, or 2) because of their lower expression, oxidative stress in T1D cannot be compensated by antioxidant enzymes. This would lead to even higher oxygen toxicity of T1D in the 1st trimester placenta.

Izvorni jezik
Engleski

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