Naslov
Fully integrated L-malic acid production process

Autori
Pandurić, Nikola ; Fabek, Diana ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2011, 53-54

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
L-malic acid; fumaric acid; Saccharomyces cerevisiae; in situ product separation

Sažetak
Bioconversion of fumaric acid to L-malic acid was performed using biocatalyst fumarase from whole permeabilized baker’s yeast cells Saccharomyces cerevisiae in a continuous stirred tank reactor, with biomass recirculation and in situ product separation. The process of in situ product separation consisted of five processing steps: biotransformation, the crystallization with acidification, sedimentation, concentration using electrodialysis and dissolution by neutralization. Experiments were conducted in a continuous stirred tank reactor, with biomass recirculation at different initial concentrations of fumaric acid (cF, U = 50, 100, 200 and 400 mmol dm-3 and T = 0.576 days). Conversions of fumaric acid achieved in the steady state were 96.15%, 91.14 %, 80.22 % and 70.36 %. Second set of experiments was conducted in a continuous stirred tank reactor, with biomass recirculation for different retention times (T = 0.299, 0.576 and 0.830 days) with the initial fumaric acid concentration of 400 mmol dm- 3. Conversions of fumaric acid achieved in the steady state were 59.57 %, 62.95 % and 51.13 %. Continuous process with in situ product separation was conducted at fumaric acid concentration of 400 mmol dm-3 and for retention time of T = 0.576 days. The overall process conversion with in situ product separation was 84.99 %.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
Ovaj rad financijski je potpomognula Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.

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