Naslov
Genetički i okolišni čimbenici varijabilnosti N-glikana u plazmi čovjeka
(Genetic and environmental determinants of variability in human plasma N-glycans)

Autori
Knežević, Ana

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
26.11

Godina
2010

Stranica
122

Mentor
Lauc, Gordan

Ključne riječi
starenje; tjelesna masnoća; nasljeđivost N-glikoma; obilježavanje sa više fluorofora; pušenje; stabilnost N-glikoma; varijabilnost N-glikoma
(aging; body fat; heritability of N-glycome; multiple fluorophores labelling; smoking; stability of N-glycome; variability of N-glycome)

Sažetak
Studies searching for human plasma N-glycans that change as a result of specific condition or disease were previously done on a small number of samples due to limitations of analytical methods. Recent improvement of analytical methods enabled our first large scale study on human plasma N-glycome. Results from almost 2000 individuals revealed high natural variability with magnitude that exceeds some previously described changes associated with diseases. The heritability of glycans was found to vary widely across individual glycan species. Effects of age, smoking, dietary habits and some other environmental factors were shown to have significant influence on specific glycan levels. On a smaller number of subjects, repeatedly sampled on a several occasions, stability of the N-glycome was determined in a single individual which leads to conclusion that N-glycome has a large biomarker potential. By involving multiple fluorophore labelling we have introduced a new approach enabling the increase of throughput from threefold on HPLC up to 36 fold on UPLC.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA