Naslov
Genotoxic effects of green tea extract on human laryngeal carcinoma cells in vitro

Autori
Durgo, Ksenija ; Kostić, Sandra ; Gradiški, Katarina ; Komes, Draženka ; Osmak, Maja ; Franekić, Jasna

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 62 (2011), 2; 139-146

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cell line; cytotoxicity; DNA damage; epicatechin gallate; epigallocatechin gallate; green tea; glutathione; lipid peroxidation

Sažetak
Green tea (Camellia sinensis) contains several bioactive compounds which protect the cell and prevent tumour development. Phytochemicals in green tea extract (mostly flavonoids) scavenge free radicals, but also induce pro-oxidative reactions in the cell. In this study, we evaluated the potential cytotoxic and pro-oxidative effects of green tea extract and its two main flavonoid constituents epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) on human laryngeal carcinoma cell line (HEp2) and its cross-resistant cell line CK2. The aim was to see if the extract and its two flavonoids could increase the sensitivity of the cisplatin-resistant cell line CK2 in comparison to the parental cell line. The results show that EGCG and green tea extract increased the DNA damage in the CK2 cell line during short exposure. The cytotoxicity of EGCG and ECG increased with the time of incubation. Green tea extract induced lipid peroxidation in the CK2 cell line. The pro-oxidant effect of green tea was determined at concentrations higher than those found in traditionally prepared green tea infusions.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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