Naslov
Suborganismic effects of formalin and temephos to earthworm Eisenia andrei

Autori
Velki, Mirna ; Hackenberger Kutuzović, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Young Environmental Scientists (YES) meeting "Environmental challenges in a changing world" : abstracts / - , 2011

Skup
Young Environmental Scientists meeting (2 ; 2011)

Mjesto i datum
Aachen, Njemačka, 28.02.2011. - 02.03.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
temephos; formalin; acetylcholinesterase; oximes; catalase

Sažetak
Since the removal of organochlorine insecticides from use, oganophosphate insecticides have become the most widely used group of insecticides in the world. Besides the target organisms, organophosphates also affect many other non-target organisms, including earthworms. Therefore, earthworms are often used for ecotoxicological research and in biomonitornig for assessing the physiological impact of exposure to organophospahtes. Since formaldehyde solutions are often used in the field as standard earthworm expellants, earthworms can be exposed to formalin in natural environment due to its use in sampling procedure. So the aim of this study was to investigate effects of exposure to organophosphate temephos alone and in combination with formalin using earthworms from Eisenia andrei species as model organisms. Suborganismic effects were examined analysing the activity of enzymes acetylcholinesterase (AChE) and catalase (CAT). Since natural occurring oximes could take part in maintaining the steady state of AChE activity in organism, amount of oximes was also measured. In first experiment earthworms were exposed to temephos (2 h) and then transferred to formalin (15 min). In second experiment earthworms were first exposed to formalin (2 h) and then transferred to temephos (2 h). As expected, both formalin and temephos caused decrease in AChE activity. More interestingly, when formalin exposure (15 min) followed temephos exposure (2 h), significant increase in AChE activity was recorded. But when earthworms were first exposed to formalin (2 h) and then to temephos (2 h) this effect was absent. Similar results were obtained when amount of oximes was measured. Also, measurement of CAT activity showed that temephos alone causes decrease in activity but in combination with formalin causes increase in CAT activity. Obtained results showed that exposure to formalin during the sampling (or from other sources) could change effects caused by exposure to temephos and therefore lead to misinterpretation of the obtained results.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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