Naslov
Aqueous two-phase extraction of polyphenols using macro- and micro-extractor system

Autori
Šalić, Anita ; Rukavina, Ivana ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2011, 69-70

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
polyphenols; aqueous two-phase systems; micro-extractor; macro-extractor

Sažetak
The aqueous two – phase systems (ATPSs) and extraction (ATPE) have been recognized as superior and versatile, preparative and analytical tools for the downstream processing of biomolecules. Up to now, several different ATPS processes have been developed for different biological products such as cells, viruses, RNA, plasmids, monoclonal antibodies and proteins but not for polyphenols. Polyphenols constitute one of the most numerous and ubiquitous groups of plant metabolites and currently, more then 8, 000 phenolic compounds are described. They have several industrial applications, such as in the production of the paints, paper, cosmetic, medicine and food industry as additives. Extraction is one of the most common methods used for isolation of polyphenolic compounds and it is mainly performed with organic solvents. These extraction procedures are efficient but the extracts are not safe for human consumption due to the potentially toxic effect from the residual solvents. Therefore, a great effort is being made in investing and developing new separation techniques. The objective of the present study was to investigate applicability of the aqueous two-phase systems for polyphenol extraction. Continuous extraction of the polyphenol from model solution and juices (wine and orange juice) in a micro- extractor and macro-extractor was performed in order to replace the generally applied batch extraction process and to demonstrate the applicability of these types of devices for ATPS supported extraction of polyphenols. Extraction efficiency for each applied system was determined and comparison has been made to find optimal method for polyphenol extraction.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj rad financijski je potpomognula Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.

POVEZANOST RADA