Naslov
Immobilization of baker's yeast cells within microchannels of different origins

Autori
Purić, Jelena ; Šalić, Anita ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2011, 65-66

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microchannels; immobilization; baker's yeast; alcohol dehydrogenase; hexanal

Sažetak
Reactions performed in microreactors are becoming more and more interesting due to high productivity and economic efficiency of those processes. High surface to volume ratio, well-defined reaction times and conditions, laminar flow, the possibility to automate the process and the concept of numbering-up instead of the scale-up are just few of numerous advantages of microreactor systems. However, not much work has been done in the development of microreactor systems for production purposes using biological catalysts, especially taking into account the sustainability of biocatalytic processes. There have been only few reports on enzyme-based transformations in microreactors and even less with whole cells even though in some cases cells can have longer lifetime then enzymes when used in biocatalysis processes. In this work baker's yeast cells were immobilised within a microchannel (d = 50 µm) and a PTFE tube (d = 1000 µm) using covalent immobilisation principles. In order to improve mass transfer in and out of the whole cells through they membrane, the cells were permeabilized with detergent before immobilisation. Enzymatic oxidation of hexanol to hexanal using whole immobilized and permeabilized yeast cells was performed in both microchannel systems and obtained results were compared to results obtained in microreactor when free cells were used as catalyst.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj rad financijski je potpomognula Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.

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