Naslov
NAD+ coenzyme regeneration in microchannel - kinetics parameters estimation and modeling

Autori
Ivanković, Mia ; Ferk, Elvira ; Šalić, Anita ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2011, 32-33

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
coenzyme regeneration; microreactor; kinetics; mathematical model of the process

Sažetak
Coenzyme NAD+ (nicotinamide adenine dinucleotide) is used in numerous biocatalytic oxidation reactions where NAD+ is reduced to NADH (reduced form of nicotinamide adenine dinucleotide). As the price of coenzyme NAD+ is extremely high, it is essential to regenerate the reduced form of coenzyme back into the oxidized form. There are several common methods of regeneration of coenzyme. In this study regeneration of coenzyme NAD+ was carried out by reversible oxidation of ethanol to acetaldehyde using enzyme alcohol dehydrogenase (ADH). Regeneration was performed in microreactor - reactor with micrometer dimensions. By using microreactor, number of advantages over conventional macroreactors were achieved such as intensifying mass transfer, reducing the consumption of chemicals, energy and time required for conducting reactions. Based on experimental results, by measuring dependence of the reaction rate on the concentration of all components of the reaction system, kinetics of coenzyme regeneration was estimated and the mathematical model of the process was developed. Validation of developed mathematical model of the process was carried out on a series of independent experiments. Based on results and good agreement between model predictions and experimental results, obtained model simulation results could be used for further process optimization and development of new microreactors.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj rad financijski je potpomognula Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.

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