Naslov
Immobilization of enzyme on superparamagnetic nanoparticles coated with chitosan

Autori
Hojnik Podrepšek, Gordana ; Sudar, Martina ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa ; Primožič, Mateja ; Knez, Željko ; Habulin, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, M. ; Primožič, M. - Maribor : Tiskarna tehniških fakultet Maribor, 2011

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
magnetic nanoparticles; chitosan; immobilization of enzymes

Sažetak
Biotechnology has undoubtedly been one of the major growth areas in science and engineering over the last ten to fifteen years. Chitosan is the N- deacetylated product of chitin, which forms an integral part of the outer shell of crustaceans and insects. It is non-toxic, hydrophilic, biocompatible, biodegradable and antibacterial and includes the presence of hydroxyl and amino groups in its structure. As the chitosan and its derivates are widely used in medicine, pharmacy and biotechnology, we decided to prepare carboxymethylated gamma-Fe2O3 chitosan nanoparticles for immobilization of biological units. Chitosan has been used as a stabilizing agent of gamma-Fe2O3 nanoparticles. Magnetic nanoparticles were prepared of maghemite by coprecipitating iron(II) and iron (III) ions. Gamma-Fe2O3 were dissolved in a solution of paraffin, Span 80 and chitosan in acetic acid with 25 % solution of glutaraldehyde. Chitosan- superparamagnetic were obtained using magnetic settling, rinsed with deionized water and ethanol and dried at 50 °C. Different concentrations of enzyme for enzyme immobilization were prepared on gamma-Fe2O3 carboxymethylated chitosan nanoparticles in phospate buffer and added to nanoparticles. Gamma-Fe2O3 chitosan nanoparticles with immobilized enzyme were collected and separated by magnetic sedimentation. Characterization of nanoparticles and the efficiency of binding the enzyme to gamma-Fe2O3- chitosan magnetic nanoparticles were confirmed by Fourier tranformation infrared (FTIR) analysis. We used transmission electron microscope (TEM) to see morphology and size of gamma-Fe2O3-chitosan nanoparticles conjugate with and without the enzyme. Efficiency of enzyme immobilization was determined by calorimetric methods of measuring the amount of protein in the supernatant after the immobilization process.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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