Naslov
Aldol addition of dihydroxyacetone to N-Cbz-3- aminopropanal

Autori
Petranović, Marko ; Sudar, Martina ; Primozic, Mateja ; Habulin, Maja ; Knez, Željko ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7the Meeting of Students and University Professors from Maribor and Zagreb / Habulin, M. ; Primožič, M. - Maribor : Tiskarna tehniških fakultet Maribor, 2011

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7the Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aldolase; N-Cbz-3-aminopropanal; kinetic model; alcohol dehydrogenase

Sažetak
Aldol addition of dihydroxyacetone (DHA) to N-Cbz- 3-aminopropanal (aldehyde) was studied in this paper. The reaction was catalyzed by D-fructose-6- phosphate aldolase from genetically modified E. coli (FSA A129S). It is a reverse reaction whose equilibrium is shifted to the side of aldol production. A part of this research is related to alcohol dehydrogenase (ADH) and coenzyme regeneration methods, considering that the substrate in this reaction, aldehyde, can be obtained by ADH catalysis from the corresponding alcohol. The reaction kinetics was determined in the case of aldol addition of DHA to aldehyde, but not for the reverse reaction. The initial reaction rate method was used. The influence of DHA and aldehyde was examined and from the gathered data kinetic parameters were estimated (Vm1, Km, DHA, Km, aldehyde). Since it was stated that a part of this research is related to ADH and coenzyme regeneration methods, the influence of different compounds that appear in the coenzyme regenerating systems were tested. It was important to see which compounds were inhibiting and which were not, because the choice of the regenerating method depends on that as well. From these data inhibition constants were estimated. The NAD+ regenerating systems investigated were: pyruvate → lactate, D-glucono- 1, 5-lactone → glucose and acetone → isopropanol. Aldol addition of DHA to N- Cbz-3-aminopropanal was carried out in the batch reactor at equimolar conditions. 80% of substrate conversion was achieved. A repetitive batch experiment was also carried out to examine the operational stability of aldolase.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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