Naslov
Cascade biocatalytic synthesis

Autori
Valek, Ivana ; Sudar, Martina ; Primozic, Mateja Habulin, Maja ; Knez, Željko ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, M. ; Primožič, M. - Maribor : Tiskarna tehniških fakultet Maribor, 2011

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oxidoreduction; aldol addition; aldolase; alcohol dehydrogenase; cascade reaction

Sažetak
In this work a cascade reaction of oxidoreduction and aldol addition was studied. The reaction scheme is presented in Figure 1. In the first reaction N-Cbz-3-aminopropanol is oxidized to N- Cbz-3-aminopropanal, which simultaneously reacts in the second reaction – aldol addition. Both reactions are equilibrium reactions. The first reaction is catalyzed by alcohol dehydrogenase (ADH) and the second one is catalyzed by D- fructose-6-phosphate aldolase (FSA A129S). Since ADH is an enzyme that requires coenzyme NAD+ (H), it is necessary to perform its regeneration. Aldolases belong to a group of enzymes called lyases and they catalyze carbon-carbon bond formation [1]. The aldol product of this reaction is used as precursor in the production of fagomine, a naturally occurring iminosugar with remarkable biological properties [2]. The kinetics of the reaction was determined by using the initial reaction rate method. The mathematical model consisting of kinetic and mass balances in the batch reactor was developed and validated in the batch reactor.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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