Naslov
Aldol addition of dihydroxyacetone (DHA) to N-Cbz- glycinal

Autori
Česnik, Morana ; Valinger, Davor ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa ; Kurtanjek, Želimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, M. ; Primožič, M. - Maribor : Tiskarna tehniških fakultet Maribor, 2011

ISBN
978-961-248-298-5

Skup
2nd Conference on Applied biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aldolna adicija ; N-Cbz-glicinal ; dihidroksiaceton ; D-fruktoza-6-fosfat aldolaza
(aldol adition ; N-CBZ-glycinal ; dihydroxyacetone ; D-fructose-6-phosphate aldolase)

Sažetak
Aldol addition is a reaction catalyzed by enzymes aldolases and cells use it for the formation of C- C bonds. In this work a novel reaction of aldol synthesis by aldol addition of dihydroxyacetone (DHA) to protected aldehide (N-Cbz-glicinal) catalyzed by enzyme D-fructose-6-phosphate aldolase (FSA A129S) [1] is presented. Addition reactions change the number of atomes or groups bounded to C-atomes. They happen on multiple bonds and are very frequent in metabolism (eg. Citryc acid cycle or carbohydrate methabolism). In this work D-fructose-6-phosphate aldolase derived from genetically modified E. coli (FSA A129S) was used which is functionally distinct from known aldolases in its tolerance of different donor substrates as well as acceptor substrates [2]. Kinetics of aldol adition of dihydroxyacetone to N-Cbz-glicinal was determined by initial reaction rates method. Measurements were conducted in batch reactor and the reaction was monitored by high performance liquid chromatography. From experimental data kinetic parameters of the model were estimated. Mathematical model was validated in batch reactor.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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