Naslov
Catehol oxidation in microreactor

Autori
Bregović, Valentina ; Tušek, Ana ; Kurtanjek, Želimir ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Conference on Applied Biocatalysis and 7th Meeting of Students and University Professors from Maribor and Zagreb / Habulin, Maja ; Primožič, Mateja - Maribor : Tiskarna tehniških fakultet Maribor, 2011, 1-6

ISBN
978-961-248-298-5

Skup
2nd Conference on "Applied Biocatalysis" and 7th Meeting of Students and University Professors from Maribor and Zagreb

Mjesto i datum
Maribor, Slovenija, 07.11.2011. - 08.11.2011

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microreactor; laccase; catehol oxidation

Sažetak
Laccases are multi-copper proteins with covalently bound carbohydrate moiety (10–45 %) that contribute to greater stability of the enzymes. They catalyze the oxidation of a wide range of substrates by radical-catalyzed reaction mechanism with a corresponding reduction of oxygen to the water in four electron transfer process. Laccases are considered environmentally friendly enzymes and lately there has been great interest in there use for the transformation and degradation of phenolic compounds. In this work enzymatic oxidation of catehol using commercial laccase from Trametes versicolor was performed in continuously operated tubular microreactor (with internal volume of 6 µL) and polytetrafluoroethylene (PTFE) tubular reactor (with internal volume of 235.62 µL) both with two inflows (“Y” shaped) and one outflow. Experiments were performed with dissolved enzyme and with covalently immobilized enzyme on the surface of the microreactor (d = 150 µm) and PTFE microchannel (d = 1000 µm). Effect of substrate aeration with air and oxygen were analysed. Using dissolved enzyme the highest conversion of X = 20 % was reached after  = 20 s in microreactor experiment where catehol solution was aerated with pure oxygen. Immobilisation stability was analysed in continuous experiment for 10 day. Conversion of catehol in experiments performed with immobilized laccase in both types of reactor configuration used, microreactor and PTFE microchannel respectively, decreases with time.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj se tekst temelji na radu koji je financirala Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske.

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