Naslov
Acute RSV infection is associated with lower TLR8- mediated IFN-gamma production in NK cells

Autori
Bendelja, Krešo ; Vojvoda, Valerija ; Turkalj- Kljajić, Mirjana ; Tešović, Goran ; Rabatić, Sabina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of the Austrian Society for Allergology and Immunology (OGAI), Book of Abstracts / - Beč, 2010, 28-28

Skup
Annual Meeting of the Austrian Society for Allergology and Immunology (OGAI)

Mjesto i datum
Beč, Austrija, 03.12.2010. - 05.12.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSV; TLR8; ssRNA; IFN-gamma; NK cells; infants

Sažetak
Toll-like receptors provide first line of defense able to activate immune system upon pathogen challenge. Respiratory syncytial virus is ssRNA virus which can cause severe lower respiratory tract infection in infants. TLR8 recognizes viral ssRNA and initiates cascade of antiviral mechanisms necessary to limit virus spread and development of specific immune reaction. NK cells are early responders in viral infections with ability to directly recognize and kill infected cells. Since peripheral blood NK cells express TLR8, we postulate that lower TLR8 receptor expression could be related to inability of NK cells to control RSV replication, to induce IFN- gamma production and to participate in development of effective anti-RSV immune response in infants. In our study we determined TLR8 expression in peripheral blood NK cells from RSV-infected infants during acute infection, healthy infants and adults. Functional status of TLR8 in NK was assessed in vitro by intracellular IFN-gamma staining upon TLR8-specific stimulation. RSV- infected infants had lower percentages of TLR8 expressing NK cells than healthy infants and adults. Lower TLR8 levels in NK cells during acute RSV infection were accompanied by lower TLR8- mediated IFN-gamma production in vitro that couldn't be corrected by exogenous IL-12/IL-8 supplementation. Moreover, expression levels of TLR8 in NK cells negatively correlated with severity of infection, assessed as hospitalization duration. In conclusion, lower expression of TLR8 in NK cells from RSV infected infants could imply inadequate engagement of antiviral innate immune mechanisms responsible for RSV replication control and generation of effective immune response that might lead to exaggerated inflammation frequently observed in infants.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Dodijeljena stipendija za pohađanje sastanka

POVEZANOST RADA