Naslov
Identification and characterization of Lactobacillus brevis L62 proteins after prolonged period of starvation

Autori
Butorac, Ana ; Cindrić, Mario ; Dodig, Ivana ; Mrvčić, Jasna ; Stehlik-Tomas, Vesna ; Bačun-Družina, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and abstracts / Power of microbes in industry and environment 2010 / Frece, J., Kos, B., Mrša, V. - Zagreb : Hrvatsko mikrobiološko društvo, 2010, 33-33

ISBN
978-953-7778-00-2

Skup
Power of Microbes in Industry and Environment

Mjesto i datum
Malinska, Hrvatska, 22.09.2010. - 25.09.2010

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lactobacilus brevis

Sažetak
In order to survive during the growth in a prolonged stationary phase in batch culture non-sporulating bacteria express wide variety of stress-response genes and alternative metabolic pathways. The lactic acid bacteria (LAB) are associated with various plant, animal and human niches and have ability to produce lactic acid during homo- or hetero-fermentative metabolism. Greater understanding of how LAB cells undergo change during prolonged starvation may facilitate new strategies for development of cell lines with advantageous traits. The purpose of this work was to examine the influence of long term incubation in nutrient wasted environment on Lactobacillus brevis L62 gene expression. The cells were grown for 75 days in spent MRS medium and 75-day-old isolates proteome was examined and compared with parent cells. Differential display estimated by 1-D and 2-D gel electrophoresis followed by MALDI-TOF/TOF protein identification revealed the key differences responsible for bacteria long-term survival in a hostile environment (deprivation of nutrition and detritus as growth media). Using two-dimensional electrophoresis, we quantitatively detected about 30 protein spots and identified 2 distinct proteins. Metabolic pathway changed on the proteome level could be successfully reconstructed by the data collected from the protein expression differences. A novel technique of de novo sequencing applied for the first time on presented model of Lactobacillus brevis revealed subtle differences in bacterial protein homology. Each variation is connected to different gene expression accomplished through the protein/peptide de novo sequencing.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA