Naslov
Determination of natural moisturizing factor in human stratum corneum by liquid chromatography

Autori
Đapić, Irena ; Kežić, Sanja ; Kammeyer, Arthur ; Jakaša, Ivone

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th International School of Ion Chromatography : Book of abstracts / Ukić, Šime ; Bolanča, Tomislav - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2011, 35-35

ISBN
978-953-6470-54-9

Skup
International School of Ion Chromatography (12 ; 2011)

Mjesto i datum
Zagreb, Hrvatska, 30.06.2011. - 01.07.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
natural moisturizing factor; atopic dermatitis; filaggrin; histamine; liquid chromatography

Sažetak
Skin is the largest human organ and its vital role is to protect body from the environmental effects. Stratum corneum, the outermost layer of the skin, is the primary barrier preventing body from water loss and ingress of pathogens and allergens. Filaggrin (filament-aggregating protein) is a key structural protein required for proper epidermal barrier function responsible for the mechanical strength and integrity of the stratum corneum. Filaggrin is proteolytically degraded into pool of free amino acids including histidine and glutamine which are further degraded into 2-pyrrolidone-5-carboxylic acid and urocanic acid. Approximately 50% of natural moisturizing factor (NMF), retained in matured corneocytes of the stratum corneum, is made of free amino acids and their derivatives. NMF is found to have substantial part in regulating hydration of the stratum corneum, pH of the skin, lipid synthesis, diffusion permeabillity etc. We developed a simple RP-HPLC method using UV detection for determination of free amino acids and their metabolites in the stratum corneum which comprize more than half of total NMF including 2-pyrrolidone-5-carboxylic acid, histidine, tyrosine, trans- and cis- urocanic acid, phenylalanine and biogenic amine, histamine. The method is based on non-invasive the stratum corneum detachment by tape stripping and subsequent extraction of tape strips in concentrated ammonia. After evaporation of ammonia, the residue is reconstituted in deionized water, filtered and injected into HPLC. The advantage of this method is ommiting the use of non-volatile solvent for extraction and derivatization procedure. The method showed to be reproducible and sensitive enough to determine above mentioned NMF components in the stratum cornuem of normal skin and skin of atopic dermatitis patients

Izvorni jezik
Engleski

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