Naslov
Determination of optimal extraction parameters of mulberry leaves using Response Surface Methodology (RSM)

Autori
Radojković, Marija ; Zeković, Zoran ; Jokić, Stela ; Vidović, Senka

Izvornik
Romanian biotechnological letters (1224-5984) 17 (2012), 3; 7295-7308

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Morus alba; mulberry leaves; extraction; response surface methodology; total phenolics; total flavonoids; antioxidant activity

Sažetak
The effects of solvent concentration (ethanol/water, 40–80%, v/v), temperature (40 – 80°C), and liquid-solid ratio (10–30 ml/g, solvent volume per g of raw material) such as their interaction on extractability of total phenolics and total flavonoids, and antioxidant activity of mulberry leaves were studied. The optimal conditions for the phenolics and flavonoids extraction, and antioxidant activity were determined by response surface methodology. The Box–Behnken design showed that polynomial regression models were in good agreement with the experimental results with the correlation coefficients of 0.9850, 0.9004 and 0.9858 for total phenolics, total flavonoids content and antioxidant activity, respectively. The experimental values agreed with those predicted, thus indicating suitability of the used model and the success of response surface methodology in optimizing the extraction conditions of investigated system. This is the first report on optimizing of extraction technology of phenol compounds and antioxidant activity of mulberry plants grown in Serbia. This information will be of considerable value to the commercial producers of mulberry trees in the country.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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