Naslov
Modelling and kinetic parameter estimation of NAD+ coenzyme regeneration in microreactor

Autori
Šalić, Anita ; Tušek, Ana ; Ivanković, Mia ; Ferk, Elvira ; Kurtanjek, Želimir ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
1st European Congress of Applied Biotechnology

Mjesto i datum
Berlin, Njemačka, 25.09.2011. - 29.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
coenzyme regeneration; microreactor; kinetics; mathematical model of the process

Sažetak
Biotransformations, using either the whole cells or isolated enzymes as biocatalysts, have been extensively applied. Mild, environmental friendly operating conditions, high specificity, efficiency and product purity is what makes these processes so interesting. Despite these advantages, the use of enzyme in industrial application has been limited by several factors, mainly high cost of the enzyme and availability in small amounts. The use of the NAD+ or NADP+ dependent dehydrogenase in the biotransformation is additionally limited by the price of coenzyme, since it should be added in a stoichiometric amount. So an efficient and economical cofactor regeneration system is necessary. Various methods are developed for this purpose (chemical, biochemical, photochemical, electrochemical or enzymatic). Among them, the most employed are enzymatic methods. Another new technology that is gaining important place in different fields of biotechnology from scientific research to industrial production and that is holding the potential to revolutionize chemical synthesis, is microreactor technology. Large surface to volume ratio, excellent mass and heath transfer, shorter residence time, smaller amount of reagents, catalyst and waste products comparing to equivalent bulk reactions, laminar flow, effective mixing and better process control, small energy consumption and less environmental impact are just some of the microsystem advantages. Aim of this work was to implement microreactor technology and enzymatic methods for coenzyme regeneration in microchannel. Enzyme alcohol dehydrogenase isolated from baker yeast was used for the NAD+ regeneration in the reaction of acetaldehyde oxidation to ethanol. Reaction kinetic was measured and mathematical model was developed. Simulation results of the developed model of acetaldehyde oxidation were compared with those obtained during the batch oxidation in a conventional macroreactor. Finally, the model prediction results were proven and verified on a set of experiments performed in a microreactor.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

Napomena
Ovaj rad financijski je potpomognula Nacionalna zaklada za znanost, visoko školstvo i tehnologijski razvoj Republike Hrvatske. Ovaj rad osvojio je nagradu za najbolji poster u kategoriji: "Bio-based products, biocatalysis and biotransformation”

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