Naslov
Utjecaj bisperoksovanadij-1,10-fenantrolina na rast i diferencijaciju tumorskih stanica u kulturi
(Effect of bisperoxovanadate-1,10-phenantroline on growth and differentiation in tumor cell lines)

Autori
Cerovac, Željka ; Kovač, Višnja ; Ban, Jasna

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Šesti kongres biologa Hrvatske / Hrvatsko biološko društvo - : Hrvatsko biološko društvo, 1997, 128-129

Skup
Šesti kongres biologa Hrvatske

Mjesto i datum
Opatija, Hrvatska, 22.09.1997. - 26.09.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
bisperoksovanadij-1;10-fenantrolin; inhibitor fosfotirozin fosfataze; diferencijacija HL60 stanica
(bisperoxovanadate-1;10-phenantroline; phosphotyrosine phosphatase inhibitor; HL60 cell differentiation)

Sažetak
Bisperoxovanadate-1,10-phenantroline, bpV, is an insulin mimetic in cultured cells. This compound activated the insulin receptor kinase and inhibited dephosphorilation of autophosphorilated insulin receptors. This compound is the most potent phosphotyrosine phosphatase inhibitor described to date. bpV induced differentiation determined by counting NBT positive cells and inhibited proliferation of HL-60 human leukemic cells in dose dependent manner (IC50 = 4 mM). At a bpV dose of 1.3 mM 50% of the cells differentiated. After 6 days of incubation at a bpV dose of 3 mM 82.1% of cells were differentiated. Under our culture conditions the doubling time of HL-60 cells was 30.7 h; addition of bpV (3 mM) increased doubling time to 48 h. In rat hepatoma 3924A cells bpV inhibited proliferation in a time- and dose- dependent fashion IC50 = 0.5 mM after 72 h of incubation. bpV and quercetin in combination are additivelly cytotoxic in hepatoma 3924A cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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