Naslov
Optimization of extraction of phenolic compounds and antioxidant activity from black mulberry leaves: A Response Surface Methodology Approach

Autori
Radojković, Marija ; Zeković, Zoran ; Jokić, Stela ; Vidović, Senka ; Lepojević, Žika ; Milošević, Svetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th International Congress of Food Technologists, Biotechnologists and Nutritionists / - , 2011

Skup
7th International Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Opatija, Hrvatska, 20.09.2011. - 23.09.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
black mulberry leaves; response surface methodology; total phenols; total flavonoids; antioxidant activity

Sažetak
Mulberry (Morus nigra L.) leaves, bark and branches have been used in traditional medicine such as diuretic, hypoglycemic and hypotensive activities. The mechanism of their effects is correlated with the content of active components. The extraction of active components from the natural sources depends on different factors. The knowledge of the effects of different extraction parameters is useful for the optimization of the process, as well for the ability to predict the extraction yield. The aim of this study was to examine the influence of solvent concentration (ethanol/water, 40–80%, v/v), temperature (40–80°C) and liquid-solid ratio (10–30 ml/g, solvent volume per g of raw material) to the total yield of phenols, flavonoids and antioxidant activity of black mulberry leaves. Response surface methodology (RSM) was used to determine the optimum extraction conditions and to investigate the effect of different variables on the observed properties of mulberry leaves. Experimental values of total phenols were in the range from 18.63 to 48.70 mg ECA/g of dried leaves and for total flavonoids in the range from 6.01 to 21.41 mg ER/g of dried leaves. The results showed a good fit to the proposed model (R2>0.90). The optimal condition to obtain the highest total phenolic and flavonoid yields were within the experimental range. The experimental values agreed with those predicted, thus indicating suitability of the used model and the success of response surface methodology (RSM) in optimizing of the investigated system extraction conditions.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA